摘要
目的 :测定黄芪注射液中黄芪甲苷的含量。方法 :采用RP HPLC法 ,流动相为乙腈 水 磷酸 (1∶2∶0 .1) ,检测波长为2 0 3nm。结果 :线性方程为Y =7.89× 10 4 X +1.16× 10 4 ,r =0 .9992 ,黄芪甲苷在 1.2 6～ 12 .6 μg范围内线性关系良好 ,平均回收率为 94.7%,RSD %为 2 .6 %(n =6 )。结论 :建立的含量测定方法简便。
OBJECTIVE To develop a water for determination of astragoloside Ⅳ in the astragale injection.METHODS Using RP HPLC,the mobile phase consisted acetonitrile water phosphoric acid (1∶2∶ 0.1 ), the detection wavelength was 203 nm.RESULTS The regression equation was Y= 7.89 ×10 4X+ 1.16 ×10 4, r= 0.999 2 , ( 1.26 ～ 12.6 μg). The average recovery was 94.71% , RSD = 2.6% ( n=6 ).CONCLUSIONS The method was simple and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2001年第4期212-213,共2页
Chinese Journal of Hospital Pharmacy
