摘要
研究了鸡菌 (Terminomycesalbuminusos)液体培养过程中 4种不同培养基对菌体得率、菌体多糖和菌体氨基氮含量的影响 ,并对其进行动力学研究。测定了菌体中蛋白质和氨基酸的含量 ,结果表明 :以菌体得率为指标 ,最适培养基为 :蔗糖 2 0g·L- 1,黄豆粉g·L- 1,酵母粉 15g·L- 1,菌体得率为 2 3.73g·L- 1。以菌体多糖为指标 ,最适培养基为 :花生粉 2 0g·L- 1,葡萄糖 2 0g·L- 1,酵母粉 15g·L- 1,菌体多糖为 14.2 8% ;深层培养 72h ,菌体多糖含量最高达 10 .35% ,培养至 96h ,菌体得率、菌体氨基氮含量最高 ,分别为 2 3.73g·L- 1和 1.2 5% ;菌体蛋白质含量为 2 8 2 8% ,氨基酸含量为 17.78%。
The effect of the four different media test on the dry weight, polysaccharide and amino nitrogen of the mycelium was investigated, the dynamics variation of the content was studied, the composition of the protein and the amino acid of the mycelium was analyzed. The results showed that the optimum media in the dry weight (g·L -1 )included: soybean powder 20,sucrose 20,yeast powder 15,the dry weight reached 23.73 g·L -1 ; the optimum media in the polysaccharide consisted of the peanut powder 20, glucose 20, yeast powder 15, the polysaccharide content was 14.28%; When liquid culturing at 72 h,the polysaccharide content reached the peak value of 10.35%, at 96 h the dry weight and the amino nitrogen content got the highest level of 23.73 g·L -1 and 1.25%; the protein content of the mycelium was 28.28% and the amino acids was 17.78%.
出处
《浙江农业学报》
CSCD
2001年第2期91-93,共3页
Acta Agriculturae Zhejiangensis
基金
浙江省自然科学基金资助项目
