摘要
目的 :观察胰腺组织及其癌变标本中基质金属蛋白酶 (MMPs)及其金属蛋白酶的组织抑制物 (TIMPs)基因表达的变化与胰腺癌临床特征、生物学行为的关系。 方法 :采用 Northern- blot、原位分子杂交 ISH的方法 ,对15例胰腺癌中 MMP2、MMP9和 TIMP1基因的 m RNA水平进行了检测 ;并就其临床意义进行分析。 结果 :胰腺癌组织 MMP2、MMP9和 TIMP1的基因表达水平明显高于正常胰腺组织。它们在胰腺癌组织和间质细胞中均有分布 ;其表达与肿瘤的大小、CA19- 9无关 ,与包膜侵犯、腹腔淋巴结转移和病理分型等胰腺癌侵袭转移的特征有密切的联系。 结论 :在胰腺癌组织中 MMPs和 TIMPs的异常表达 ,有助于临床医师对患者预后判断。
Objectives:To evaluate the potent role of matrix metalloproteinases(MMPs) and its tissue inhibitors(TIMPs) in the processes of metastasis and local invasiveness of Chinese human ductal adenocarcinoma of the pancreas, and to evaluate the possible biologic association between its gene expression and the clinical manifestations. Methods: Northern blot and in situ hybridization has shown MMPs and TIMPs gene expression in the pancreas and alterations associated with neoplastic transformation. Fifteen cases of surgical pancreatic specimens were examined with cDNA probes to MMP2, MMP9 and TIMP1, findings were correlated with the size of tumor section, CA19 9, pathological classification, thrombosis and infiltration of capsule and lymph nodes. Results: Increased levels of genes mRNA from MMP2, MMP9 and TIMP1 were found in the examined pancreatic cancer tissues, MMP2≈MMP9<TIMP1, whereas low levels of transcripts for MMP2, MMP9 and TIMP1 were detectable in pancreata of organ donors. Transcripts coding for MMP2, MMP9, TIMP1 were found in both stroma and tumor cells. Gene expression of MMP2, MMP9 and TIMP1 has shown an obvious correlation with the infiltration of capsule cells, surrounding lymph nodes and the specific histopathological features. Conclusions: Imbalance between MMPs and TIMPs may help physicians to assess the metastatic potential and the prognosis of individual patients.
出处
《医学研究生学报》
CAS
2001年第2期117-120,共4页
Journal of Medical Postgraduates
