摘要
目的：观察胰腺组织及其癌变标本中基质金属蛋白酶（ＭＭＰｓ）及金属蛋白酶组织抑制因子（ＴＩＭＰｓ）基因的表达，以探讨ＭＭＰｓ及ＴＩＭＰｓ在肿瘤侵袭和转移过程中的作用。方法：采用ｃＤＮＡ探针（ＭＭＰ２、ＭＭＰ９、ＴＩＭＰ１）对两株胰腺癌细胞（ＰａＴｕ８９８８、ＰａＴｕ８９０２）、１５例胰腺癌手术切除标本和８例正常胰腺组织标本进行Ｎｏｒｔｈｅｒｎ杂交。结果：胰腺癌组织和ＰａＴｕ胰腺癌细胞ＭＭＰ２、ＭＭＰ９和ＴＩＭＰ１的基因表达水平明显高于正常胰腺组织，其趋势为：ＭＭＰ２≈ＭＭＰ９＜ＴＩＭＰ１。结论：在胰腺癌组织中，ＭＭＰ２、ＭＭＰ９、ＴＩＭＰ１可能参与了组织成分较强的降解反应过程，从而使肿瘤更易侵袭和转移；肿瘤和间质细胞似乎都能产生ＭＭＰｓ和ＴＩＭＰｓ。
Objective: To evaluate the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human pancreatic tissue and pancreatic cancer specimens in order to explore the role of MMPs and TIMPs in the neoplastic transformation and invasiveness. Methods: Two pancreatic cancer cell lines (PaTu8988, PaTu8902), 15 cases of surgical pancreatic cancer specimens and 8 normal pancreatic tissue specimens were tested by Northern blot with cDNA probes (MMP2, MMP9 and TIMP1). Results: The expression of MMP2, MMP9 and TIMP1 genes in pancreatic cancer tissue and PaTu pancreatic cancer cell line was significantly higher than that in normal pancreatic tissue and its trend was MMP2≈MMP9<TIMP1. Conclusion: The results suggest the potent role of MMPs and TIMPs in the processes which lead to metastasis and local invasiveness of human pancreatic cancer cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第5期442-444,共3页
Academic Journal of Second Military Medical University
基金
全军医药卫生科研基金
关键词
基质金属蛋白酶
抑制因子
胰腺肿瘤
MRNA
matrix metalloproteinases
tissue inhibitors of metalloproteinases
gene expression
