摘要
目的 探讨结核菌L型感染能否引起生精细胞凋亡 ,从而推测结核菌L型感染与男性不育的关系。方法 用牛型结核分支杆菌L型反复直接注入成年雄鼠睾丸内 ,40天后取睾丸组织分别作HE染色及凋亡细胞的原位末端标记显示(ISEL)。结果 实验组 2 0例雄性小鼠有 15例曲细精管的生精细胞有凋亡 ;对照组 2 0例无一例有生精细胞凋亡 ;两者生精细胞凋亡有显著性差异 (P <0 .0 0 5 )。结论 结核菌L型感染可引起小鼠生精细胞凋亡 ;结核菌L型感染与男性不育可能有一定关系。
Aim To detect if tubercle bacillus L-form can induce apoptosis of spermatogenic cells, and guess the relation between infection of tubercle bacillus L-form and male infertility.Methods After injecting tubercle bacillus L-form to rat testis repeatedly,testis tisuss was taken to have HE staining,meanwhile apoptotic cells were detected with in situ end-labeling(ISEL). Results Apoptosis was found in 15 cases of 20 experimental rat testis;there is no apoptosis in the control group;apoptosis has manifest deference between the two groups(P<0.005).Conclusion Infection of tubercle bacillus L-form can cause spermatogenic cell apoptosis,perhaps there is some relation between infection of tubercle bacillus L-form and male infertility.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2001年第3期58-59,21,F002,共4页
Chinese Journal of Zoonoses
关键词
结核菌L型
细胞凋亡
不育症
原位末端标记
小鼠
Tubercle bacillus L-form
Infection
Spermatogenic cell
Apoptosis
Infertility
In situ end ledeling
