摘要
为建立一种可分泌人胶质细胞源性神经营养因子 (GDNF)的工程细胞 ,研究其在帕金森病基因治疗中的可能作用。克隆携带 Kozak序列的人 GDNFc DNA,并转染至人类神经母细胞瘤细胞系 SH- SY5 Y细胞 ,将此工程细胞与大鼠原代多巴胺能神经元共培养 ,免疫组化检测多巴胺能神经元。发现工程细胞可防止多巴胺能神经元退变死亡 ,有助于多巴胺能神经元抵抗 MPP+ 的毒性损伤。表明可分泌人
To construct a kind of engineered cells that can secrete human glial cell line-derived neurotrophic factor(GDNF)and study its possible effects on gene therapy of Parkinson’s diseaseCloned the GDNFcDNA with Kozak sequence by RT-PCR,and then transfected it into SH-SY5Y cell line,co-cultured the engineered cells with primary mesencephalic cellsResults showed that the engineered cells showed obvious protecive effects such as anti-degeneration and anti-MPP+lesionGDNF produced by the engineered cells was of full biological functionIt suggests that the engineered SH-SY5Y cells can secrete human GDNF and could play an important role in gene therapy of Parkinson’s disease
出处
《山东医药》
CAS
北大核心
2001年第7期1-2,共2页
Shandong Medical Journal
基金
国家自然科学基金! (NO.3 9480 0 8)
