摘要
以加工番茄“87- 5”为材料 ,采用反转录PCR(RT -PCR)技术将加工番茄PG基因的cDNA序列克隆到pGEM -T载体上 ,并进行了全序列测定分析。结果表明 ,加工番茄的PG基因的cDNA与国内外报道的番茄PG基因的cDNA ,在碱基序列及氨基酸序列上均有差异。
The entire cDNA encoding polygalacturonase was successfully cloned with RT-PCR technique from pericarp of ripe processing tomato fruits “87-5”,and its whole nucleotide sequence was analysized.The result showed that there was difference between the sequence of PG cDNA and that of amino acids among processing tomatoes and tomatoes reported.It was proved that PG gene in tomatoes had polymorphism.
出处
《生物技术》
CAS
CSCD
2001年第2期1-4,共4页
Biotechnology
