摘要
目的 研究经用氦氖激光针刺治疗帕金森病患者针刺前后尿液中多巴胺 (DA)的含量变化 ,尝试建立评价该种传统医学理论、方法与现代技术相结合的全新疗法的客观量化标准。方法 尿液经有机溶剂提取 ,HPLC 荧光检测法。结果 DA在 2 0～ 40 0ng·mL-1范围内与峰高线性关系良好 ,r=0 .9996。方法回收率为 ( 89.6 9± 3.95 ) %～ ( 92 .99± 6 .5 3) %。患者体内DA含量与帕金森病体征紧密相关。未用多巴类药物的患者针刺前DA含量均低于健康人。结论 HPLC RF测定人尿液DA含量的方法 。
OBJECTIVE To determine the concentrations of dopamine (DA) in human urine,studying the change of uric dopamine content before and after laser acupuncture treated Parkinsons disease patients.To estabilsh attemptly the standard of apprasing the treatment method which is combined traditional medical theory with modern technology.METHODS The urine samples were extracted with organic solvent and determined by HPLC RF.RESULTS A good correlation was obtained between dopamine over 20～400 ng·mL -1 and peak height, r =0.9996.The recoveries of DA were (89.69±3.95)%～(92.99±6.5)% for urine samples.There was a close correlation between DA content and the severity of parkinsons disease.The DA content in urine of patients before acupuncture was less than healthy ones.DA content in urine increased most at 4 h and content still maintained high as normal scope at 12 after acupuncture.CONCLUSION This HPLC RF assay was sensitive,accurate stable and convenient.It is an important method to evaluate the effects of treated Parkinsons disease by laser acupunctrue.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第4期269-271,共3页
Chinese Pharmaceutical Journal
