摘要
目的 探讨前列腺癌雄激素不敏感性的发生。方法 用细胞免疫化学方法和流式细胞术定位、定量分析体外培养的人前列腺癌雄激素不敏感细胞株PC 3m和雄激素敏感细胞株LNCaP中主要热休克蛋白 70 (HSP70 )的表达。结果 2种不同生物学特性的细胞株表面及细胞内均有HSP70表达 ,但PC 3m细胞膜及细胞内的阳性染色程度高于LNCaP ;流式细胞术测定的PC 3m细胞膜及细胞内的表达量 (17.61± 4.2 0 ,69.5 4± 10 .5 0 )分别高于LNCaP(7.42± 1.70 ,2 1.83±6.3 0 ) ,两者比较差异均有显著性 (P <0 .0 5 )。结论 正常状态下雄激素不敏感前列腺癌细胞株过表达HSP70 ,可能与雄激素不敏感性的发生。
Objective To study the emergence of androgen insensitivity in prostatic cancer. Methods Heat shock protein 70 (HSP70) expression in androgen insensitive cell line PC 3m and androgen sensitive cell line LNCaP of prostatic cancer in vitro culture was located and quantitated using cytoimmunochemistry and flow cytometry. Results Membranous and intracellular expression of HSP70 were detacble in PC 3m and LNCaP, however, surface, cytoplasmic or nuclear HSP70 positivity of PC 3m were far more intensive than those of LNCaP by cytoimmunochemistry. Meanwhile the amount of membranous and intracellular HSP70 expression in PC 3m(17.61±4.20, 69.54±10.50 respectively) was higher than that in LNCaP(7.42±1.70, 21.83±6.30 respectively)with the differences being significant ( P <0.05) by flow cytometry. Conclusion Under normal condition, androgen insensitive prostatic cancer cell line PC 3m overexpressed HSP70, which may be responsible for the emergence and development of androgen insensitivity in prostatic carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第2期169-170,共2页
Chinese Journal of Experimental Surgery
