摘要
目的 观察阿魏酸钠 (sodiumferulate ,SF)对离体肝癌细胞生长的抑制作用。方法 将人EBL 740 4肝癌细胞以 1× 10 8/l浓度接种于 2 5cm2 细胞培养瓶中 ,接近单层时 ,分为SF组和对照组。SF组用含SF(终浓度为 1mg/l)和小牛血清 (5 0ml/l)的 16 40培养液培养细胞 ,对照组用含小牛血清 (5 0ml/l)的 16 40培养液培养细胞 ,48h后 ,收集细胞 ,碘化丙啶 (propidiumiodide ,PI)染色后 ,用流式细胞仪检测每一细胞周期的DNA含量 ,并根据DNA的含量分类细胞。 结果 (1)SF组细胞凋亡为 10 .5 % ,对照组为 5 .9% ,凋亡被上调。 (2 )SF组DNA百分比在S期明显降低 (SF组为 33 .12 % ,对照组为 47.83 % ) ,在G2 /M期明显增加 (SF组为 2 7.87% ,对照组为 15 .0 4% )。结论 SF诱导了人EBL 740 4肝癌细胞凋亡 ,抑制了人EBL 740 4肝癌细胞的增殖 ,SF能是一种有效抑制肝癌生长的中药。
Objective To demonstrate the inhibitory effects of sodium ferulate (SF) medicine on human EBL 7404 hepatocellular carcinoma cells. Methods Human EBL 7404 hepatocellular carcioma cells at a density of 1×10 8 cells/l in 25cm 2 flasks were divided into SF and control groups. Cells of SF group was further incubated in 1640 medium cotaining SF(1g/L) and FCS(50mL/L), while cells of the control group was further incubated in 1640 medium containing FCS(50mL/L). After 48 hours, cells of SF and control groups were collected respectively and stained with propidium iodide (PI). The DNA content in each phase of cells cycle was determined by flow cytometry, The number of cells in each phase (G 0/G1, S and G 2/M) were analyzed by DNA content.Results 1. The percentage of apoptotic cells of SF group was 10.5, that of the control group was 5.9, an up regulation of apoptosis could be observed. 2. The number of cells of SF group in S phase decreased significantly as compared with control group (33.12%, 47.83% respectively), whereas the number of cells of SF group in G 2/M phase increased significantly (27.87%, 15.04%, respectively). Conclusion SF induced apoptosis of human EBL 7404 hepatocellular carcioma cells and inhibited proliferation of these cells obvioulsy. SF could be a potent herb medicine in inhibition growth of liver cancer.
出处
《肝脏》
2001年第1期19-20,共2页
Chinese Hepatology
