摘要
给雄性Wistar大鼠sc不同剂量的镉金属硫蛋白(CdMT),结果表明镉接触组尿镉、尿钙和尿蛋白浓度都高于对照组,并与镉剂量间存在剂量效应关系,而血清游离钙浓度无变化;镉接触组肾皮质钠泵和钙泵活性低于对照组,体外试验结果也显示镉能抑制钙泵活性,谷胱甘肽(GSH)和半胱氨酸对这种抑制有保护作用}镉接触组肾皮质GSH含量低于对照组,而丙二醛(MDA)含量则高于对照组,高剂量组肾皮质cAMP/cGMP比值低于对照组。肾皮质钙泵活性、cAMP/cGMP比值、GSH含量三者都与尿钙浓度间呈负相关,MDA含量则与尿钙呈正相关,揭示镉引起的钙尿症是由肾重吸收钙障碍造成的,可能与镉引起的肾脏钠泵、钙泵、环核苷酸、GSH和脂质过氧化等改变有关。
Twenty-four male Wistar rats were divided into 4 groups and injected subcutaneously with cadmium-metallothionein (CdMT) in the doses of 0, 0.3, 0.6, 0.9 mg Cd / kg respectively. Upon exposure to different dose of CdMT, the dose-dependent increasing of urinary Cd, Ca and protein were observed 12 h after CdMT injection, but there was no change in serum free Ca. In the groups exposed to CdMT, Na+, K+-ATPase (Na pump) and Ca2+-ATPase (Ca pump) activities in kidney cortex were significantly inhibited. A concentration-dependent inhibition of Ca pump activity induced by CdCl2 in vitro study was also shown, but it could be protected in the presence of glutathione (GSH) or L-cysteine. A lower content of GSH and a higher content of malondialdehyde (MDA) in kidney cortex were observed in the groups treated with CdMT, as compared with the control group. In the highest dose of CdMT group, cAMP/cGMP ratio was lower than that in the control group. The Ca pump activity, cAMP / cGMP ratio and GSH content in kidney cortex were negatively correlated with the concentration of urinary Ca. The MDA content in kidney cortex was positively correlated with urinary Ca. The results suggested that the changes of Na pump and Ca pump, cyclic nucleotide, GSH, and lipid peroxidation (LPO) induced by CdMT in the kidney would be involved in the disturbance of renal Ca reabsorption which causes calciuria characteristic of the Cd nephrotoxicity.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1991年第4期306-311,共6页
Chinese Journal of Pharmacology and Toxicology
基金
The project was supported by Science Foundation of Guizhou Province, Medicine 89-3064
关键词
镉金融硫蛋白
尿钙
蛋白尿
谷胱甘肽
丙二醛
环核苷酸
大鼠
肾脏
药理
cadmium-metallothionein
calciuria
proteinuria
glutathione
malondialdehyde
cyclic nucleotide
rat
kidney
