摘要
目的 研究非甾体抗炎药吲哚美辛 (indomethacin)和美洛昔康 (meloxicam)对细菌脂多糖 (LPS)诱导表达的C5 7小鼠腹腔巨噬细胞核转录因子NF κappaB(NF κB)的抑制作用。 方法 NF κB的测定采用电泳迁移率改变法。结果 小鼠腹腔巨噬细胞经LPS诱导后细胞NF κB含量明显增高。Indomethacin和meloxicam在 10 -5- 10 -7mol·L-1浓度下可明显降低LPS激活的小鼠腹腔巨噬细胞NF κB活化。结论 Indomethacin和meloxicam对NF κB活化的抑制作用可能是两者的抗炎作用机理之一。
AIM To study the inhibitory effects of indomethacin and meloxicam on NF κB from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice. METHODS NF κB was measured with the method of electrophoretic mobility shift assay (EMSA). RESULTS After induction by LPS at the concentrations of 1 and 3 μg·mL -1 , the NF κB content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10 -7 -10 -5 mol·L -1 , decreased the activation of NF κB at the concentrations of 1 and 3 μg·mL -1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 μg·mL -1 . CONCLUSION The inhibitory effects of indomethacin and meloxicam on NF κB activation may be one of their mechanisms of antiinflammatory actions.
出处
《药学学报》
CAS
CSCD
北大核心
2001年第3期161-164,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金! (39870 90 7)
