摘要
目的 探讨雄激素受体基因 CAG微卫星多态性与前列腺癌以及前列腺增生的关系。方法 运用聚合酶链反应 -单链构象多态性分析法 (PCR- SSCP) ,对 18例良性前列腺增生、37例前列腺腺癌组织进行雄激素受体基因 CAG微卫星数量测定。结果 良性前列腺增生、B期、C～ D期前列腺癌、高分化、中分化以及低分化前列腺癌的雄激素受体基因 CAG均数分别是 2 6 .11± 3.35、2 5 .0 4± 1.88、2 2 .14± 2 .6 4、2 5 .6± 1.36、2 4.2 9± 3.19和 2 3.6 4±2 .6 4。其均数比较 ,良性前列腺增生与 C～ D期前列腺癌以及低分化前列腺癌间差异有显著性 (P=0 .0 0 1～0 .0 16 ) ,良性前列腺增生与 B期前列腺癌、中分化及高分化前列腺癌其差异无显著性 (P=0 .2 49～ 0 .6 36 )。结论 雄激素受体基因 CAG微卫星数量的差异性可能与良性前列腺增生以及不同类型前列腺癌的发病机制相关。
Objective To observe CAG microsatellites of androgen receptor(AR) gene in prostate cancer(PC) and benign prostatic hyperplasia(BPH).Methods The number of CAG microsatellites in exon 1 of the AR gene was measured in tissues of 18 cases of BPH and 37 of PC by polymerase chain reaction single strand conformation polymophism(PCR SSCP) analysis.Results The averages CAG repeat numbers were 26.11±3.35 in BPH,25.04±1.88 in stage B of PC,22.14±2.64 in stage C～D of PC,25.6±1.36 in high,24.29±3.19 in middle and 23.64±2.64 in low differentiation of PC tissues,respectively.The averages CAG repeat numbers in BPH were significantly higher than those in the stage C～D of PC( P =0.001) and in the low differentiation of PC( P =0.016),whereas there were no differences among those of BPH,stage B of PC,the middle and high differentiation of PC( P =0.249~0.636).Conclusion The pathogenesis of PC and BHP should be associated with the polymorphism of CAG microsatellites in AR gene.\;
出处
《实用肿瘤杂志》
CAS
北大核心
2001年第2期86-89,共4页
Journal of Practical Oncology
