摘要
目的探讨胃癌组织中雄激素受体(AR)基因和蛋白的表达及临床意义。方法用免疫组化SP法检测胃癌组织AR蛋白的表达,运用RT—PCR方法检测胃癌组织中AR—mRNA的表达水平。结果胃癌组织AR阳性率为47.6%,癌旁组织AR阳性率为61.9%,两组比较有显著性差异;AR在胃癌中的阳性表达与肿瘤的组织类型、分化程度、淋巴结转移情况以及TNM分期有关。AR基因在胃癌组织和配对的癌旁组织中表达量分别为0.752±0.140和0.326±0.216,差异有统计学意义(P<0.01)。结论AR、AR基因阳性表达的胃癌具有性激素依赖性,检测胃癌组织中的AR基因及其蛋白对临床判定预后及进行内分泌治疗有一定的指导意义。
Objective To study the expression of androgen receptors （AR） in gastric cancer tissue and its clinical significance. Method The expression of AR in gastric cancer was detected by immunohistochemical SP method. The mRNA level of AR in gastric cancer tissue was detected by PT - PCR. Results The positive rate of AR was 47. 6%, while that of adjacent tumor tissue was 61.9% （P 〈0. 05）. The positive expression in gastric cancer was correlated with the tissue type of tumor, the grade of differentiation, the shift situation of lymph node, and TNM stages. The expression quantity of AR gene in gastric cancer tissues and paired tissues adjacent to gastric cancer was （0. 752±0. 140） and （0. 326±0. 216） （P 〈 0. 01 ）. Conclusion AR-positive gastric cancer is sex hormone - dependent. The detection of AR of gastric cancer is useful to guide clinical prognosis and endocrine-based treatment.
出处
《癌症进展》
2008年第5期514-517,共4页
Oncology Progress
