摘要
本文研究了腐乳生产菌种梨形毛霉所产蛋白酶对大豆分离蛋白的降解条件 ,并分析了酶解产物的疏水性、分子量分布及氨基酸组成。结果表明该蛋白酶酶促降解大豆分离蛋白的最佳条件是 :温度 4 5℃、反应时间 5h、pH6 .0、酶用量 750U ;在此条件下制备的大豆肽经质谱检测其分子量在 10 0 0D左右 ,完全符合大豆肽产品的要求 ;经品尝 ,该产品无任何苦涩味 ,色泽淡黄 ,无需脱盐处理 。
The degradation conditions of isolated soybean protein with protease from Mucor piriformis Fischer and the hydrophobicity,distribution of molecular weight and amino acids composition of the hydrolysate have been studied.The results showed the best degradation condition of soybean protein isolated as 45℃,pH6.0,enzyme 75OU,reaction time 5h.The molecular weight of the enzymatic decomposition product was 1000 Dal,analyzed by Mass spectrum.the product was yellowish without any bitter and astringent odour.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2001年第1期55-58,共4页
Journal of the Chinese Cereals and Oils Association
基金
湖南省教委科研项目
