摘要
目的探讨磷酸化视网膜母细胞瘤蛋白(phospho-retinoblastoma protein,p-Rb)在苯并[a]芘致神经元凋亡过程中的作用。方法用CCK-8试剂检测细胞活性;Annexin-V/PI双染法检测细胞凋亡率,免疫荧光细胞化学染色观察p-Rb表达,免疫印记法(Western-blot)检测p-Rb的表达情况。结果与对照组相比,各染毒组神经元活性降低(P<0.05),凋亡率增高(P<0.05),p-Rb表达量增高(P<0.05),2μmol/L组加入抑制剂后细胞活性增强(P<0.05),凋亡率下降(P<0.05),p-Rb阳性细胞数减少(P<0.05),p-Rb表达量降低(P<0.05)。结论苯并[a]芘可引起磷酸化Rb水平升高,是苯并[a]芘致神经元凋亡的可能机制。
[ Objective]To explore the role of phospho-Rb （p-Rb） in cortical neurons apoptosis induced by benzol [a]pyrene （B[a]P）. [Methods] The cell viability was determined by Cell Counting Kit 8. Cell apoptotic rates were detected by Annexin-V/PI assay. Immunofluorescence staining was used to observe the expression of p-Rb. The expression of p-Rb was detected by western-blot. [ Results] Compared with the control group, the neuronal viability in poisoned groups were decreased （P 〈 0.05}, apoptotic rates were increased （ P 〈 0.05 ）, the expressions of p-Rb in each group were increased （ P 〈 0. 05 ）. The 2 μmol/L group with the addition of inhibitive showed the enhanced cell viability （ P 〈 0. 05 ） , decreased apoptotic rates （ P 〈 0. 05 ） , decreased number of p-Rb positive cells （ P 〈 0. 05 ）, and the decreased expressions of p-Rb （ P 〈 0.05 ）. [ Conclusion] B [ a ] P can induce the rising of expression level of p-Rb, which may be a probable mechanism of neuron apoptosis induced by B[ a] P.
出处
《职业与健康》
CAS
2014年第13期1776-1779,共4页
Occupation and Health
基金
国家自然科学基金(项目编号:81072279)
