摘要
目的:评价过氧化物酶体增殖物激活受体β/δ(PPARβ/δ)的激动剂GW501516对人角质形成细胞(KC)增殖、迁移和粘附的影响。方法:体外培养正常人KC,采用MTT比色法及体外计数法,观察正常人KC在不同浓度GW501516(0,1,5,10,25,50,100 ng/ml)作用下,其增殖、迁移及粘附情况。结果:与对照组比较,GW501516促进KC增殖(P<0.01),呈剂量依赖性;GW501516显著促进了KC的迁移(P<0.001);细胞粘附性与对照组间无统计学差异(P>0.05)。结论:GW501516能够促进人KC增殖和迁移,对KC粘附无影响。
Objective：To investigate the influence of peroxisome proliferator receptorβ/δ（ PPARβ/δ） agonist GW501516 on the proliferation migration and attachment of cultured human keratinocytes （ KC） . Methods：After the KCs were treated with different concentrations of GW501516 （ 0 1 5 10 25 50 100 ng/ml） the cell proliferation migration and attachment were measured by MTT method. Results：Compared with control group GW501516 significantly increased the cell proliferation （ P〈0.001） in a dose-dependent manner and markedly promoted the cell migration （P〈0.001）.There was no significant difference in the cell attachment between the experimental group and control group （ P〉0.05） . Conclusion：The results demonstrate that GW501516 can stimulate human KC proliferation and migaration but has no effect on KC attachment.
出处
《中国麻风皮肤病杂志》
2014年第6期323-326,共4页
China Journal of Leprosy and Skin Diseases
基金
广东省自然科学基金项目(编号:S2012040006694)
广东省医学科研基金项目(编号:B2012329)
深圳市科技计划项目(编号:201203035)
