摘要
目的 探讨荧光原位杂交（FISH）三探针联合检测在儿童急性B淋巴细胞白血病（B-ALL）诊断中的应用价值.方法 对207例B-ALL患儿全部行三探针（TEL-AML1、BCR-ABL、MLL） FISH联合检测和常规染色体核型分析.结果 207例B-ALL患儿经FISH三探针联合检测,151例（72.9％）检出异常,包括典型阳性信号44例、非典型信号148例（其中41例为多探针同时检出异常）.常规染色体核型分析技术检出染色体异常53例（25.6％）.结论 对初诊的儿童B-ALL患者采用三探针联合FISH检测可以有效提高遗传学异常检出率.
Objective To evaluate the three-probe fluorescence in situ hybridization （FISH） panel in the diagnosis of pediatric B cell acute lymphoblastic leukemia （B-ALL）.Methods Three-probe （TEL-AML1,BCR-ABL and MLL） FISH and conventional cytogenetic analysis were performed in 207 children with B-ALL.Results In 207 B-ALL children,the three-probe FISH panel assay showed that 151 cases carried genetic aberrancies with a positive rate of 72.9%,including 44 cases with typical positive signal patterns and 148 cases with atypical signal patterns （among them 41 cases have multiprobe abberancy）.The conventional cytogenetic analysis detected 53 cases chromosomal abnormality with a positive rate of 25.6%.Conclusion The detection rate of genetic abnormalities in newly-diagnosed pediatric B-ALL could be significantly improved by using three-probe FISH panel upon conventional cytogenetic analysis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2014年第6期542-545,共4页
Chinese Journal of Hematology
关键词
原位杂交
荧光
儿童
白血病
B细胞
诊断
In situ hybridization, fluorescence
Children
Leukemia, B-cell
Diagnosis
