摘要
目的:研究生存素(survivin)对紫外线(UV)诱导的肿瘤细胞DNA损伤修复作用及其机制。方法:本研究构建和包装survivin蛋白shRNA慢病毒载体;依据逐孔稀释法确定转染效率及滴度;以实时荧光定量法确定干扰效果;采用宿主细胞复活法(HCR)检测survivin蛋白表达对细胞整体DNA损伤修复能力的影响。结果:干扰survivin的表达显著降低肺腺癌H1299细胞对UV诱导DNA损伤的修复能力。结论:Survivin在UV诱导DNA损伤的修复作用中扮演重要角色。本研究可以为以survivin为靶点的肿瘤治疗提供一定的理论依据。
AIM： To detect the DNA damage repair effect and its mechanism mediated by survivin during UV-induced apoptosis in tumor cells. METHODS： This study constructed and packaged lentiviral vector for survivin shRNA. Transfection efficiency and titer were measured by the dilution method. Real-time fluorescence quantitative method was applied to identify interference effects. Host-cells reactivating assay （HCR） was adopted to evaluate impact of survivin on cellular DNA damage repair capacity.RESULTS. Interference of survivin significantly reduced cellular DNA damage repair on lung adenocarcinoma H1299. CONCLUSION. Survivin plays an important role in DNA damage repair. Our research provides evidence for cancer therapy targeting survivin.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2014年第5期497-502,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金项目(81272485)
安徽省自然科学基金项目(1408085MH163)
皖南医学院中青年自然科学基金项目(WK2012F09)
