摘要
利用室内选育 119代的抗杀虫双和抗杀螟丹小菜蛾Plutellaxylostella品系和敏感品系 ,通过反复回交建立近等基因系。用Operon公司合成的 80个引物对抗杀虫双近等基因系和抗杀螟丹近等基因系小菜蛾基因组DNA进行PCR扩增 ;在抗杀虫双近等基因系中有 3个引物分别产生了 1条特异扩增带 ,2个引物分别产生了 2条特异扩增带 ,1个引物产生 3条特异扩增带 ;在抗杀螟丹近等基因系中 ,有 4个引物分别产生了 1条特异扩增带 ,1个引物产生了 2条特异扩增带。在培育近等基因系的多次回交过程中 ,与抗性有关的遗传因子被逐步置换到敏感品系的基因组中 ,因此可以认为这些特异带与小菜蛾对杀虫双和杀螟丹的敏感性有关。
Two near isogenic lines (NILS) of \%Plutella xylostella\% were cultivated by back crossing for 12 successive generations between its resistant and susceptible strains to dimehypo and cartap in the laboratory. DNA from NILS was compared using optimized randomly amplified polymorphic DNA (RAPD) methods.Of the 80 primers tested,6 primers produced 1 or more special bands in NILS of dimehypo resistance (DR), but none in the susceptible strain,and 7 primers produced 1 or more special bands in NILS of cartap resistance (CR).By repeated back crossing,the gene related to resistance was displaced to susceptible genome.Therefore,it is thought that these special bands throughout the genome in this study are likely to represent 1 or more loci that influence susceptibility to dimehypo and cartap in the diamond\|back moth.
出处
《昆虫学报》
CAS
CSCD
北大核心
2001年第1期15-20,共6页
Acta Entomologica Sinica
基金
国家自然科学基金资助项目!(39770 5 0 4)
