摘要
目的观察蜂毒明肽对阿尔茨海默病(AD)小鼠认知功能的影响。方法 10周龄昆明种雌性小鼠60只,随机分成6组,每组10只,分别为正常对照组、假手术组、AD模型组、蜂毒明肽低剂量组、蜂毒明肽中剂量组、蜂毒明肽高剂量组。正常对照组不做任何处理,假手术组小鼠侧脑室注射生理盐水。采用侧脑室注射β-淀粉样肽25-35(β-amyloid protein,Aβ25-35)法构建AD小鼠模型。造模14 d后,蜂毒明肽干预的各组小鼠分别腹腔注射不同剂量的蜂毒明肽,连续5 d。Morris水迷宫测试认知功能,免疫组化染色观察海马CA1区Aβ25-35的表达。结果与正常对照组相比,蜂毒明肽各组和AD模型组小鼠逃避潜伏期时间较长,目标象限停留时间较短,穿越平台次数降低,海马CA1区Aβ25-35阳性表达增强(P均<0.05),而假手术组无统计学差异(P均>0.05)。蜂毒明肽中、高剂量组小鼠与AD模型组小鼠相比,前者逃避潜伏期有所缩短,目标象限停留时间增加,穿越平台次数有所增多,海马CA1区Aβ25-35阳性表达有所减低(P均<0.05)。结论蜂毒明肽能提高AD小鼠认知功能,减少海马CA1区Aβ25-35的表达。
Objective To observe the effects of apamin on cognitive function in mice with Alzheimer＇s disease （AD）. Methods Sixty Kunming female mice aged 10 weeks were randomly divided into 6 groups （ n = 10 each） ： control group, sham-operation group, AD model group, apamin low, medium and high dosage groups. No treatment was given in normal control group. Normal saline was injected into lateral cerebral ventricle in shamoperation group. AD mice model were established by injecting β-amyloid protein （ Aβ25_35 ） into lateral cerebral ventricle. After 14 days, intraperitoneal injection of different dose of apamin for 5 days was given in apamin low, medium and high dosage groups. Morris water maze test was used to investigate the effects of apamin on cognitive function, and then immunohistochemistry staining was used to observe the expression of Aβ25 -35 at hippocampus CA1 region. Results Compared with normal control group, the mice of apamin low,medium,high dosage groups and AD model group had longer escape latency period, shorter target quadrant standing time, fewer number of quadrant entries, higher expression level of Al325_35 at hippocampus CA1 region （ all P 〈 0. 05 ）, while aforementioned indexes in sham-operation group remained unchanged （ all P 〉 0.05 ）. Compared with AD model group, the mice in apamin medium and high dosage groups had shorter escape latency period, longer target quadrant standing time, more the number of quadrant entries, lower expression level of Aβ25-35 at hippocampus CA1 region （ all P 〈 0.05 ）. Conclusions Apamin could improve the cognitive function of AD mice and reduce the expression of Aβ25 -35 at hippocampus CAI region.
出处
《中国临床研究》
CAS
2014年第6期644-646,共3页
Chinese Journal of Clinical Research
基金
广东省珠海市科技计划资助项目(20052013)
