摘要
以华南205(cv.SC205)为对照,研究7个华南系列木薯品种华南5号(cv.SC5)、华南6号(cv.SC6)、华南7号(cv.SC7)、华南8号(cv.SC8)、华南9号(cv.SC9)、华南10号(cv.SC10)、华南11号(cv.SC11)的叶绿素含量、叶绿素荧光参数及光系统Ⅱ(PSⅡ)中相关蛋白表达水平的差异。结果表明:SC6叶片叶绿素a、叶绿素b及总叶绿素含量均显著低于对照SC205及其它6个品种;叶绿素荧光参数中ΦPSⅡ显示8个木薯品种PSⅡ的实际光合效率Φ从高到低顺序为ΦSC5、ΦSC8、ΦSC9、ΦSC10、ΦSC205、ΦSC11、ΦSC7、ΦSC6。Western Blot结果表明D1蛋白、放氧复合体(OEC)及核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)均在SC6叶片中表达最低,而在SC5、SC8、SC9叶片中表达较高。叶绿素荧光参数及PSⅡ相关蛋白可为高效选育木薯优良品种提供参考。
In order to detect the chlorophyll content ,chlorophyll fluorescence parameters and protein ex-pression levels related with PSⅡ in different cassava varieties , SCATC5 （ cv.SC5 ） , SCATC6 （ cv.SC6 ） , SCATC7 （cv.SC7）,SCATC8 （cv.SC8）,SCATC9 （cv.SC9）,SCATC10 （cv.SC10）,SCATC11 （cv.SC11）, SCATC205 （cv.SC205） were studied in this experiment,and SC205 （cv.SC205） was the control as CK.The results showed that the contents of chlorophyll a ,chlorophyll b and total chlorophyll of SC 6 were significantly lower than that of the control cv .SC205 and those of the other six cassava varieties .The chlorophyll fluorescence parameters of ΦP SⅡ revealed that the actual photosynthetic efficiency order of 8 cassava varieties from high to low was ΦSC5 ,ΦSC8 ,ΦSC9 ,ΦSC10 ,ΦSC205 ,ΦSC11 ,ΦSC7 ,ΦSC6 .The western Blot results showed that the expression levels of D1 protein,oxygen-evolving complex （ OEC） and ribulose-1,5-bisphosphate carboxylase/oxygenase （Rubisco） were the lowest in cv.SC6 leaves,but higher in cv.SC5,cv.SC8,cv.SC9 leaves.Chlorophyll fluores-cence parameters and PSⅡrelated proteins can provide a reference for screening cassava varieties efficiently .
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2014年第3期514-519,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家重点基础研究发展计划(973计划)项目(2010CB126606)
国家自然科学基金项目(31371684)
中央级公益性科研院所基本科研业务费专项(PZS-062)
关键词
木薯
叶绿素荧光
光合效率
蛋白质
cassava
chlorophyll fluorescence
photosynthetic efficiency
protein
