摘要
目的研究卵胞浆内单精子注射技术(ICSI)中不同来源精子对卵母细胞受精、胚胎质量及发育潜能的影响。方法回顾性分析我中心因单纯男性因素行ICSI助孕治疗的197例患者的临床资料,按不同精子来源分为射精组(n=102)、PESA组(n=68)、TESA组(n=27)3组,其中将射精组又分为严重少弱畸精子症组(n=67)和隐匿精子症组(n=35)。比较4组间受精率、优质胚胎率、新鲜胚胎移植周期的着床率及妊娠率,以及低质胚胎继续培养的优质囊胚形成率。结果少弱畸精子射精组、隐匿精子射精组、PESA组、TESA组的正常受精率分别为64.8%、62.1%、75.6%、61.6%,其中PESA组明显高于其它3组(P<0.05),4组的优质胚胎率、新鲜胚胎移植周期的着床率及妊娠率无明显差异(P>0.05)。4组间低质胚胎继续培养的优质囊胚形成率也无显著差异(P>0.05)。结论 PESA来源的精子ICSI中正常受精率较高,但在ICSI中不同来源精子对胚胎质量及发育潜能无显著影响。
Objective To evaluate the impact of spermatozoa from different sources on normal fertilization of oocytes, embryo quality and embryo developmental potential in intracytoplasmic sperm injection （ICSI） cycles. Methods A retrospective analysis was conducted among 197 patients undergoing ICSI cycles in our center. The patients were classified into 3 groups according to the sources of semen, namely ejaculated spermatozoa group （n=102）, percutaneous epididymal sperm aspiration （PESA） group （n=68）, and testicular sperm aspiration （TESA） group （n=27）. The ejaculated spermatozoa group was further classified into oligoasthenoteratozoospermia （n=67） and cryptozoospermia （n=35） subgroups. The normal fertilization, high-quality embryo, implantation and clinical pregnancy rates were compared among the groups; the rate of high-quality blastocyst formation in in-vitro culture of non-top quality embryos was also observed. Results The patients with PESA showed significantly higher normal fertilization rate （75.6%） than those in oligoasthenoteratozoospermia （64.8%）, cryptozoospermia （62.1%）, and TESA （61.6%） groups （P〈0.05）. No significant differences were found in the high-quality embryo, implantation, and clinical pregnancy rates among the groups （P〉0.05）. The rate of high-quality blastocyst formation in the in-vitro culture of non-top quality embryos was also comparable among the groups （P〉0.05）. Conclusion Although spermatozoa obtained with by PESA is associated with a higher normal fertilization rate, the sources of spermatozoa do not significantly affect the embryonic quality and developmental potential in ICSI cycles.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2014年第6期857-861,共5页
Journal of Southern Medical University
基金
卫生行业科研专项基金(201002013)
关键词
卵胞浆内单精子注射技术
精子来源
胚胎质量
胚胎发育潜能
intracytoplasmic sperm injection
sources of spermatozoa
embryo quality
blastocyst
developmental potential,embryo
