摘要
目的观察雪莲注射液对帕金森病（PD）模型小鼠炎症损伤的保护作用。方法C57BL／6小鼠108只按随机数字表法分为阴性对照组、PD模型组、阳性对照组（丹参注射液组）、雪莲注射液高剂量组[1．04mL／（kg·d）]、中剂量组[0．52mL／（kg·d）]、低剂量组[0．26mL／（kg·d）]，每组18只。PD小鼠模型通过腹腔注射百草枯（10mg／kg）建立。各组小鼠在实验前及实验后7周行神经功能行为评估；采用高效液相法检测小鼠黑质一纹状体部位多巴胺（DA）含量；采用免疫组化染色方法检测小鼠黑质部位酪氨酸羟化酶（TH）、整合素α（mac-1）及肿瘤坏死因子-α（TNF-α）阳性细胞数。结果实验7周后，PD模型组小鼠运动协调障碍明显，雪莲注射液高剂量组能够明显缓解。高效液相法检测结果显示，PD模型组DA含量较阴性对照组明显降低，差异有统计学意义（P〈0．05）；雪莲注射液高剂量组DA的含量明显增加，达到（10．90±1．04）μg/g，与PD模型组相比差异有统计学意义（P〈0．05）。免疫组化染色结果显示：PD模型组TH阳性细胞数较阴性对照组明显降低，mac-1阳性细胞数、TNF—α阳性细胞数较阴性对照组明显增加，差异均有统计学意义（P〈0．05）。雪莲注射液高剂量组TH阳性细胞数较PD模型组明显增加，达到（65．18±6．00）个／视野：mac-1阳性细胞数及TNF-α阳性细胞数较PD模型组明显降低，分别为（93．18±5．33）个／视野、（92．73±23．21）个／视野，差异均有统计学意义（P〈0．05）。结论高剂量雪莲注射液能够明显改善百草枯所致PD小鼠的运动协调障碍，促进黑质部位DA能神经元的存活，其机制为抑制小鼠黑质部位小胶质细胞的活化及其介导的炎症反应。
Objective To investigate the effect of Xuelian injection on mice models with Parkinson＇ s disease （PD）. Methods C57BL/6 mice were given paraquat （PQ） by peritoneal injection （10 mg/kg） to induce PD models. Then, they were randomly divided into six groups： PD group, negative control group, positive control group, low-dose （0.26 mL/kg.d）, middle-dose （0.52 mL/kg.d） and high-dose （1.04 mL/kg·d） Xuelian injection groups. Pole test and open field activity test were used for assessment of behavior 7 weeks after the injection; the dopamine （DA） content in the substantia nigra pars compacta （SNc） was observed by high-performance liquid chromatography （HPLC）. The expressions of tyrosine hydroxylase （TH）, integrin α （mac-1） and tumor necrosis factor α（TNF-α） in the SNc were measured by immunohistochemical staining under optical microscope. Results Seven weeks after the injection, the movement coordination disorder was significant in the PD group, and that had partial remission in the high-dose Xuelian injection group. HPLC showed that the DA content in the PD group was significantly lower than that in the negative control group （P〈0.05）; that in the high-dose Xuelian injection group （[10.90±1.04] μg/g） was significantly higher than that in the PD group （P〈0.05）. Immunohistochemical staining indicated that PD group had significantly decreased number of TH positive cells and statistically increased numbers of mac-1 and TNF-α positive cells as compared with thenegative control group （P〈0.05）; high-dose Xuelian injection group had significantly increased number of TH positive cells （65.18±6.00） and statistically decreased numbers of mac-1 and TNF-α positive cells （93.18±5.33 and 92.73±23.21） as compared with the PD group （P〈0.05）, Conclusions High-dose Xuelian injection can obviously improve the movement coordination disorder and survival of DA neurons in the SNc, whose protective effect might be related to the inhabitation of mi
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2014年第6期547-551,共5页
Chinese Journal of Neuromedicine
基金
新疆维吾尔自治区自然科学基金（2010211A56）
关键词
帕金森病
雪莲注射液
小胶质细胞
炎症反应
Parkinson＇s disease
Xuelian injection
Microglia
Inflammation injury
