摘要
采用 3种聚乙二醇 无机盐双水相体系 ,即聚乙二醇 硫酸盐、聚乙二醇 柠檬酸盐、聚乙二醇 磷酸盐双水相体系 ,研究成相浓度、起始牛血清清蛋白 (BSA)总浓度、外加有机溶剂及外加盐对牛血清清蛋白分配特性的影响 .对聚乙二醇 硫酸盐体系 ,改变起始牛血清清蛋白的总浓度 ,得到平衡时上、下相牛血清清蛋白浓度随起始牛血清清蛋白总浓度变化的曲线方程 ,上相宜用“饱和型”方程表示 ,下相适合用线性方程表示 。
The partion of BSA in three different aqueous two phase systems, polyethylene glycol(PEG) sulphate, citrate, and phosphate, has been investigated at different concentrations of PEG, sulphate, neutral salt, additives and at varying overall BSA concentrations. In PEG sulphate system, BSA concentration in top phase has been correlated with a “saturation” type equation and which in bottom phase has been correlated with a linear type equation. The partition coefficient could be satisfactorilly predicted, as a function of the ovarall BSA concentration, by the ratio between the “saturation” equation of the top phase and the linear equation of the bottom phase.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2001年第2期146-149,共4页
Journal of Wuxi University of Light Industry
关键词
聚乙二醇-硫酸盐
牛血清清蛋白
双水相
分配特性
PEG sulphate,PEG citrate, PEG phosphate
BSA
aqueous two phase system
partition
