摘要
目的:建立小毛茛内酯的含量测定方法,优选猫爪草中小毛茛内酯的提取工艺。方法:采用HPLC测定小毛茛内酯含量,流动相甲醇-乙腈-水(40∶40∶20),检测波长254 nm。在单因素试验基础上,以小毛茛内酯质量分数为指标,采用L9(34)正交试验考察提取时间、料液比、乙醇体积分数及粉碎粒度对提取工艺的影响。结果:最佳提取工艺为粉碎粒度80目,加3倍量70%乙醇提取3次,每次2 h;小毛茛内酯质量分数(1.24±0.22)%。结论:优选的工艺稳定可行且提取率高,为猫瓜草的开发与利用提供参考。
Objective： To establish a method for determination of ternatolide and optimize its ultrasonic extraction process from Ranunculi Ternati Radix. Method： HPLC was adopted to determine the content of ternatolide with mobile phase of methanol-acetonitrile-water （40： 40： 20） and detection wavelength at 254 nm. Based on single-factor tests, orthogonal design was used to optimize extraction technology by taking extraction time, ratio of solid to liquid, the concentration of ethanol and grinding particle size as factors. Result： Optimal ultrasonic extraction conditions were as follows： extracted thrice with three times the amount of 70% ethanol for 2 h each time, grinding particle size of 80 mesh; under these conditions, the content of ternatolide was （ 1.24 ± 0.22） %. Conclusion： Optimized extraction process was stable and feasible with high extraction yield, this study could provide a reference for development and utilization of Ranunculi Ternati Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第12期17-19,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
青海大学中青年基金项目(2012-QYY-1)
青海大学医学院大学生科技创新基金项目
关键词
猫爪草
小毛茛内酯
正交试验
提取工艺
Ranunculi Ternati Radix
ternatolide
orthogonal test
extraction technology
