摘要
目的：探讨雌激素替代治疗（estrogen replacement treatment，ERT）对卵巢切除（ovariectomy，ovx）大鼠海马CA1区有髓神经纤维及其髓鞘的影响。方法：对10～11月龄的雌性SD大鼠进行双侧卵巢切除术，术后1月将成功切除卵巢的大鼠随机分为安慰剂对照组（OVX＋O组）和雌激素替代治疗组（OVX＋E组），前者给予连续4周安慰剂（芝麻油）处理，后者给予连续4周17β-雌二醇替代治疗，4周后采用Morris水迷宫对2组大鼠的空间学习能力进行测试，用化学发光法测定2组大鼠体内循环雌激素浓度．用透射电子显微镜和体视学方法对两组大鼠海马CA1区有髓神经纤维及其髓鞘进行定量研究。结果：OVX＋E组大鼠体内循环雌激素浓度明显高于OVX＋O组（t=5．688，P=-0．000）；OVX＋E组大鼠空间学习能力明显提高（F=9．645，P=-0．006）。2组大鼠CA1区体积（t=1．451，P=0．181）、有髓神经纤维体积（t=-1．158，P=0．294）、有髓神经纤维髓鞘体积（u=-0．642，P=0．521）、有髓神经纤维平均直径（t=0．630，P=0．545）和有髓神经纤维髓鞘厚度（u=-1．746，P=-0．081）均不存在统计学差异，但是OVX＋E组直径在0．6．0．7μm的有髓神经纤维髓鞘厚度比OVX＋O组大鼠增加14．1％（t=2．428，P=0．038），OVX＋E组直径在0．8-0．9μm的有髓神经纤维直径与轴突直径差比OVX＋O组大鼠增加18．2％（t=2．432，P=-0．038）。结论：短期雌激素替代治疗可以改善大鼠空间学习能力并对海马CA1区有髓神经纤维髓鞘具有保护作用。
Objective：To investigate the effects of short-term estrogen replacement on myelinated nerve fibers and myelin sheaths in CA1 region of ovariectomized rats. Methods：Female SD rats （10-11 month-old） were bilaterally ovariectomized. One month after ovariectomy, successful ovariectomized rats were randomly divided into placebo group （OVX＋O） which received sesame oil for 4 weeks and estrogen replacement group（OVX＋E） which received continuous 17β-estradiol replacement treatment for 4 weeks. After 4 weeks, spatial learning capacities of two groups were tested using the Morris water maze. Then, the serum estradiol levels were detected by chemiluminescence method. The myelinated nerve fibers and myelin sheaths in CA1 region were quantitatively investigated by transmission electron microscope and stereological methods. Results：The serum estradiol levels were significantly increased after estrogen replacement treatment（ t=5.688,P=0.000 ）. The spatial learning capacity of OVX＋E rats was significantly improved when compared with that of OVX＋O rats（F=9.645,P=0.006）. There was no significant difference in the CA1 volume,the volume of myelinated nerve fibers,the volume of myelin sheaths,the mean diameter of the myelinated fibers and the mean thickness of myelin sheaths between two groups （P=0.181, P=0.29d, P=0.521, P=0.545, P=0.081, respectively）. The mean thickness of myelin sheaths of the myelihated fibers with diameters between 0.6 to 0.7 μm was significantly higher by 14.1% in OVX＋E group when compared with that of OVX＋O group（t=2.428,P=0.038）. The difference between the mean diameter of myelinated nerve fibers and the mean diameter of axons with the mean diameter of 0.8 to 0.9 μm in OVX＋E group was significantly increased by 18.2% when compared with that of OVX＋O group（t=2.432,P=-0.038）. Conclusions ：Short-term estrogen replacement can enhance the spatial learning capacity and can exert pro- tective effects on the myelin sheaths in the CA1 region of middle-aged ovar
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2014年第4期425-432,共8页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:811171238、31271288)
2012年重庆市“百名学术学科领军人才”培养计划资助项目
