摘要
目的观察青藤碱(sinomenine,Sin)对偏头痛模型大鼠蛛网膜下腔小血管周围炎性细胞数的影响,探讨Sin治疗偏头痛的抗炎作用。方法健康wistar大鼠随机分为空白对照组、模型组、Sin低、中、高剂量干预组,每组10只。皮下注射硝酸甘油复制实验性偏头痛动物模型,药物干预4 h后断头取脑干及其脑膜。HE染色,显微镜下观察蛛网膜下腔小血管周围炎性细胞数的变化,计数蛛网膜下腔小血管周围单位面积(以视野为单位)炎性细胞数。结果模型组大鼠蛛网膜下腔小血管周围炎性细胞(以淋巴细胞、单核细胞为主)明显增多,与空白组相比较,差异有显著性统计学意义[(28.10±2.28)vs.(1.60±1.07),P<0.01];Sin高、中、低剂量组蛛网膜下腔小血管周围炎性细胞明显减少,与模型组相比较,差异有显著性统计学意义[Sin高剂量组(2.60±1.43)、Sin中剂量组(5.10±1.37)、Sin低剂量组(9.10±0.88)vs.(28.10±2.28),P<0.01]。结论 Sin可显著减少硝酸甘油诱导的偏头痛模型大鼠蛛网膜下腔小血管周围炎性细胞数,抑制偏头痛的无菌性神经源性炎症反应,提示Sin有治疗偏头痛的作用。
Objective To examine the effects of sinomenine （Sin） on inflammatory cell numbers of subarachnoidsmall perivascular and explore the role of anti-inflammatory of Sin in the treatment of migraine in a rat model of migraine.Methods Healthy wistar rats were randomly divided into 6 groups normal group, model group and Sin low dose group, Sinmiddle dose group and Sin high dose group. The rat migraine model was established by subcutaneously administration ofnitroglycerin. The brain and mater of rats were decapitated following 4 h of medical intervention. HE staining was used tostain inflammatory cells and inflammatory cells within perivascular spaces were counted under light microscope. ResultsCompared to normal group, the inflammatory cells （mainly lymphocyte and monocyte） within perivascular spaces were in-creased significantly in model group [（28.10±2.28） vs. （1.60± 1.07）, P 〈 0.01）]; The inflammatory cells in all three Singroups were significantly reduced compared with model group [Sin high dose group （2.60± 1.43）, Sin middle dose group（5.10± 1.37）, Sin low dose group （9.10±0.88） vs. （28.10±2.28）, P 〈 0.01 ]. Conclusions Sin can significantly reduce inflam-matory cells within perivascular spaces in the rat model of migraine, suggesting that Sin can effectively treat migraine.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2014年第4期198-201,共4页
Chinese Journal of Nervous and Mental Diseases
基金
贵州省省长基金项目(黔省专合字(2008)112号)
