摘要
目的:研究移植脑源性神经营养因子(BDNF)转染的骨髓间充质干细胞(MSCs)对阿尔茨海默病(AD)大鼠N-甲基-D-天冬氨酸受体1(NMDAR1)及认知功能的影响。方法:将50只SD大鼠随机平均分为正常对照组、假手术组、AD组、MSCs组、BDNF组。正常对照组不予任何处理,其他4组制备AD模型;MSCs组和BDNF组于制模第11天分别注入10μL(约5×106)MSCs或pIRESneo-EGFP-BDNF转染的MSCs。采用Morris水迷宫测试大鼠学习记忆能力,免疫组化染色法测定NMDAR1水平。结果:MSCs组、BDNF组与AD组比较平均逃避潜伏期(AEL)明显缩短、跨平台次数显著增加;BDNF组较MSCs组改善更显著(P<0.01),NMDAR1水平更高(P<0.01)。结论:BDNF转染骨髓MSCs对AD大鼠的认知有改善作用,且促进海马区NMDAR1的表达。
ObjectiveTo study the changes of N-methyl-D-aspartate receptor 1 （NMDAR1） and learning and memory function after transplanting bone mesenchymal stem cells （MSCs） modified with brain-derived neurotrophic factor （BDNF） gene in brain of Alzheimer disease （AD） rats models. Methods： Fifty healthy SD rats were randomly divided into 5 groups with equal number in each group, groups normal, sham, AD, MSCs, and BDNF. The normal group had received no treatment; and the AD models were established in the other 4 groups. The MSDs and the BDNF groups were injected by 10 μL （about 5×10^6） MSCs or MSCs modified with BDNF gene respectively. The learning and memory abilities of the rats were evaluated with Morris water maze test. The expression of NMDAR1 was detected by immunohistochemistry. Results： The average escape latency （AEL） was significantly shorter and the frequency of crossing the platform was significantly increased in the MSCs and BDNF groups compared with those in the AD group. The rats in the BDNF group showed greater learning and memory function improvement and higher expression of NMDAR1 compared with those in the MSCs group（ P〈0.01）. Conclusion： Bone MSCs modified with BDNF gene could improve the memory and learning of AD rats and up-regulate the expression of NMDAR1 in the hippocampus.
出处
《神经损伤与功能重建》
2014年第3期195-198,共4页
Neural Injury and Functional Reconstruction
