摘要
目的:比较添加乙酰左旋肉碱的冷冻保护剂对人精子的冻存效果。方法:将30例患者的精液标本(15例正常精子与15例弱精子)液化,并PureSperm梯度离心处理后分为8组,分别为对照组(A1/A2组,仅冷冻保护剂),B1/B2组(2.5mmol/L乙酰左旋肉碱),C1/C2组(7.5mmol/L乙酰左旋肉碱),D1/D2组(15mmol/L乙酰左旋肉碱),液氮中冷冻2周,比较冻融前后各组精子存活率、活力、DNA完整性和活性氧(ROS)水平。结果:无论是正常精子组或弱精子组,精子解冻后的活力、存活率、DNA完整性较冷冻前下降,ROS水平升高。A1/A2组与B1/B2组精子解冻后的活力、存活率、DNA完整性和ROS水平比较无差异。C1/C2组和D1/D2组精子解冻后的活力、存活率、DNA完整性和ROS水平较A组明显升高,但C1/C2组和D1/D2组各项指标相比无差异。结论:玻璃化冻存对精子存在损伤,含7.5mmol/L乙酰左旋肉碱的冷冻保护剂可以提高人类精液的冻存效果。
Objective： To assess the effect of acetyl-L-carnitine on human spermatozoa after freezing-tha- wing process. Methods： Cryopreservation of human spermatozoa were added with acetyl-L-carni- tine at different concentration （group B1/B2： 2. 5 mmol/L, group C1/C2： 7. 5 mmol/L, and group D1/D2： 15 mmol/L）, and their outcomes were compared with that of control group （group A1/A2： no acetyl-L-carnitine）. The viability, motility, and DNA integrity by comet assay and the ROS level of frozen-thawed spermatozoa of each group was compared. Results. There is no significant difference of motility and viability between group A1/A2 and group B1/B2, while the motility and viability of group C1/C2 and group D1/D2 was significantly higher than that of group A1/A2. Compared with that respectively in group A1/A2, CR, TD,TL and OTM was sig- nificantly lower in group C1/C2 and group D1/D2. No difference of ROS level was found between group A1/A2 and B1/B2, while with increasing acetyl-L carnitine concentration, the ROS level in group C1/C2 and D1/D2 was significantly lower than in group A1/A2. Conclusion. Acetyl-L- carnitine is an effective antioxidant against cryo-damage on post-thawed human spermatozoa.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2014年第3期426-431,共6页
Medical Journal of Wuhan University
关键词
乙酰左旋肉碱
玻璃化冷冻
冷冻损伤
活性氧
Acetyl-L-Carnitine
Vitrification
Cryo-Damage
Reactive Oxygen Species
