摘要
目的了解我院血培养中的革兰阴性(G-)杆菌的分布及对常用抗生素的耐药情况,为合理使用抗菌药物提供依据。方法采用法国梅里埃的Bact/ALERT 3D 60血培养仪和美国BD公司PHOENIX 100全自动细菌鉴定分析仪进行培养鉴定和药敏实验。结果分离到的G-杆菌中,主要有大肠埃希菌、肺炎克雷伯菌肺炎亚种、铜绿假单胞菌等,大肠埃希菌对哌拉西林/他唑巴坦(TZP)、亚胺培南(IPM)、阿米卡星(AN)、美洛配能(MEM)的耐药率均<10%;肺炎克雷伯菌肺炎亚种对阿米卡星(AN)、美洛配能(MEM)、亚胺培南(IPM)的耐药率分别为10.5%、7.9%、5.3%;铜绿假单胞菌对庆大霉素(GM)、多粘菌素(CL)的耐药率分别为5.0%、0.0%。三种常见分离菌对其余抗生素耐药率都相对较高,有的甚至已经100%耐药。结论本院血培养中分离出的常见三种G-杆菌中耐药率严重,应引起临床重视,必须严格合理用药。
Objective To understand the gram negative blood culture in our hospital（G-） bacteria distribution and resistance to antibiotics, provide the basis for the rational use of antimicrobial agents. Methods The French bioMerieux Bact/ALERT 3D 60 blood culture system and American BD PHOENIX 100 automatic bacteria identification instrument culture identification and drug sensitive experiment. Results Isolated strains of G- bacteria, mainly Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli sensitive antibiotic piperaciUin/ tazobactam （TZP）, imipenem （IPM）, Amikacin（AN）, meropenem（MEM）, the resistance rate of less than 10%. Amikacin was sensitive to the Klebsiella pneumoniae subspecies（AN）, meropenem（MEM）, imipenem （IPM）, resistance rates were 10.5%, 7.9%, 5.3%, gentamicin was sensitive to the Pseudomonas aernginosa （GM）, polymyxin hormone （CL）, resistance rates were 5.0%, 0.0%. Three kinds of common bacteria isolated from the rest of the antibiotic resistance rate was relatively high, and some even had 100% resistance. Conclusion The blood culture isolate three common resistant G- coli rate is serious, should cause clinical attention, must strictly rational drug use.
出处
《中国现代医生》
2014年第16期76-78,共3页
China Modern Doctor
关键词
血培养
革兰氏阴性杆菌
抗生素
耐药性
Blood culture
Gram negative bacteria
Antibiotics
Drug resistance
