摘要
目的探讨重组人促红细胞生成素(rhEPO)对大鼠颅脑损伤后损伤脑组织核因子-κB(NF-κB)表达和血清肿瘤坏死因子-α(TNF-α)水平的影响。方法将90只雄性成年Wistar大鼠分为假损伤组、颅脑损伤组及rhEPO组。采用改进Feeney法制作大鼠颅脑损伤模型,rhEPO组伤后即刻腹腔注射rhEPO(3 000 IU/kg);伤后3 h、12 h、24 h、48 h、72 h、5 d和7 d免疫组织化学染色法检测NF-κB的表达、双抗体夹心酶联免疫吸附法检测血清TNF-α水平。结果伤后3 h,损伤脑组织NF-κB表达明显升高,伤后24 h达最高峰,随后其表达水平逐渐下降,至伤后7 d仍明显升高(P<0.05);rhEPO治疗后,每个时间点损伤脑组织NF-κB表达水平均明显降低(P<0.05)。伤后3 h,血清TNF-α水平也明显升高,伤后12 h迅速达到最高峰,随后其水平逐渐下降;伤后3d,其水平又再次升高,随后又逐渐下降,至伤后7 d仍明显升高(P<0.05)。rhEPO治疗后,每个时间点血清TNF-α水平均明显降低(P<0.05)。结论 NF-κB和TNF-α可能在颅脑损伤后的炎症反应中发挥重要作用,而rhEPO可能通过抑制NF-κB和TNF-α的表达而减少损伤脑组织的炎症反应,发挥脑保护作用。
Objectives To observe the changes in the expression of nuclear factor kappa B （NF-κB） in the injured cerebral tissues and the serum level of tumor necrosis factor-α （TNF-α） after traumatic brain injury（TBI） in rats and effects of r-HuEPO on them. Methods Ninety adult male Wistar rats were randomly divided into 3 groups, i.e. sham TBI group （n=6）, TBI group （n=42） and recombinant human erythropoietin （r-HuEPO） treatment group （n=42）, in which the animal were treated by r-HuEPO immediately after TBI. The experimental TBI model was established by Feeney method. The serum levels of TNF-α were determined with ELISA. The expressions of NF-κB protein in the injured cerebral tissues were determined by immunohistochemistry. Results The expression level of NF-κB and serum levels of TNF-α were significantly lower in the sham TBI group than those in TBI group 3, 12, 24, 48, 72, 120 and 168 hours after TBI （P〈0.05）, which were significantly lower than those in r-HuEPO treatment group （P〈0.05）. Conclusion It is suggested r-HuEPO may decrease the inflammatory response of the injured cerebral tissues after TBI by inhibiting the expression TNF-α and NF-κB, and exerting protective effect to neurons because the previous study showed that TNF-α and NF-κB might play a pivotal role in inflammatory reaction.
出处
《中国临床神经外科杂志》
2014年第5期290-293,共4页
Chinese Journal of Clinical Neurosurgery
