摘要
目的探讨大鼠液压脑损伤后皮层微血管损伤情况及其与伤后脑水肿的关系。方法成年SD大鼠30只,随机分为正常组(n=6)、假手术组(n=6)、损伤组(n=18),其中损伤组分为伤后6h、24h、72h三亚组,每亚组6只。利用液压冲击法建立大鼠颅脑损伤模型,显微镜下观察直接损伤侧和非直接损伤侧皮层微血管损伤情况,CD34标记血管内皮细胞评价血管密度改变,干湿重法检测脑组织含水量的变化。结果大鼠皮层微血管损伤后6h可见血管支行迂曲、扩张、充血,伤后24h可见少量血栓形成,损伤后72h可见有较多血栓形成。损伤组CD34阳性细胞数明显低于假手术组和对照组(P<0.05),而脑组织含水量明显高于假手术组和对照组(P<0.05),而后两组无统计学差异(P>0.05)。损伤组直接损伤侧皮层微血管损伤较非直接损伤组严重,而且伤后24h较伤后6、72h严重。结论颅脑损伤后脑微血管损伤为全脑性血管损伤,这可能是伤后脑水肿形成的机制之一。
Objective To investigate the changes of the cortical capillaries and brain edema after lateral fluid percussion brain injury in adult rat and their relationship. Methods Thirty adult Sprague-Dawley rats were randomly divided into three groups, i.e., normal group （n=6）, sham-operated group （n=6） and injured group （n=18）. The rat model was made by lateral fluid percussion brain injury with an impact of 2.3 kPa. The rats in injured group were killed 6, 24 and 72 hours after injury with six rats in each time point. The pathological changes of cerebral tissues were detected by HE staining. The changes of cortical capillaries were evaluated by counting the number of CD34 marked vascular endothelial cells. The brain water content was calculated by wet and dry method. Results The HE staining showed that many cortical capillaries with no congestion and endothelial ceils with normal shape could be seen in normal group and sham-operated group. Six hours after injury, the injured cortical capillaries became tortuosity and congestion; 24 hours later, a few of thromboses could be seen in the injured cortical capillaries and the thromboses significantly increased 72 hours after injury. The number of CD34 positive cells was significantly lower and the brain water content was significantly higher in injured group than those in normal group （P〈0.05） and sham-operated group （P〈0.05）, but there were no significant difference between normal group and sham-operated group （P〉0.05）. Moreover, the number of CD34 positive cells was significantly lower and the brain water content was significantly higher in the cortical tissues of the direct injured side of rats in injured group than those in indirect injured side （P〈0.05）, and the most severe injury of the cortical tissues occurred 24 hours after injury. Conclusion Cortical capillaries injury may be involved in pathogenesis of brain edema after traumatic brain injury in adult rat.
出处
《中国临床神经外科杂志》
2013年第8期485-487,共3页
Chinese Journal of Clinical Neurosurgery
基金
全军医学科研"十二五"面上课题(编号CWS11J235)
关键词
颅脑损伤
皮层微血管
脑水肿
大鼠
Traumatic brain injury
Cortical capillaries
Brain edema
Rat
