摘要
目的观察低频电磁场(Low frequency electromagnetic field,LFEMF)对毛囊生长期中再生毛囊的形态学及该过程中角质化细胞生长因子(Keratinocyte growth factor,KGF)表达的影响。方法选用雌性C57BL/6成年鼠制作毛囊生长期模型,随机分为对照组和LFEMF组。LFEMF组采用50 Hz、5 mT磁场,30 min/d,连续作用16 d。取两组小鼠脱毛处的全层皮肤,HE染色法观察毛囊生长期中毛囊形态学的改变,荧光定量PCR法和Western-blot检测KGF表达情况。结果 HE染色显示:3 d时,对照组中未见球状基质包绕毛乳头,LFEMF组球状基质部分包绕毛乳头,毛乳头上部有黑色素形成;9 d、16 d时,LFEMF组中毛囊的长度显著增加(P<0.05)。荧光定量PCR及Western-blot检测结果表明LFEMF组较对照组的KGF基因转录水平及蛋白水平均上调。结论 LFEMF能促进毛囊的生长,其机制可能与KGF的分泌增加有关。
Objective To observe the effects of low frequency electromagnetic field on the growth of hair follicles and expression of keratinocyte growth factor in the anagen of the hair follicle. Methods The female C57BL/6 adult mice were selected to make the model of the anagen of the hair follicle, and randomly divided into the control group and the low frequency electromagnetic field group which was exposed to 50Hz、5mT electromagnetic field,30min/d, for 16d.After being exposed to the low frequency electromagnetic field for D3 , D9 and D16, the full-thickness skin of the hair removal site was used to detect the changes of morphology of the hair follicle and expression of keratinocyte growth factor in the anagen of the hair follicle. Results Hematoxylin-eosin staining results showed that： at 3 d,Hair bulb partially surrounded the dermal papilla, and there was melanin above the dermal papilla in the low frequency electromagnetic field group;at D 9 and D16,the length of the regeneration of hair follicle in the low frequency electromagnetic field group was increased. Fluorogenic quantitative PCR and Western-Blot analysis showed that the gene and protein expression of keratinocyte growth factor in the low frequency electromagnetic field group was significantly up-regulated compared with the control group （P〈0.05）. Conclusions The result showed that the low frequency electromagnetic field may promote the secretion of keratinocyte growth factor to advance the growth of hair follicles in the anagen of the hair follice.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2014年第3期321-324,329,共5页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金(S2012010009498)
广东省自然科学基金(S2012010009308
S2012010009498)
广东省科技计划资助项目(2010B031600164)
关键词
低频电磁场
毛囊生长期
毛囊再生
角质化细胞生长因子
Low frequency electromagnetic field
Hair follicle growth cycle model
Hair follicle regeneration
Keratinocyte growth factor
