摘要
目的:观察隔药饼灸对功能性胃肠病(functional gastrointestinal disorders,FGIDs)肝郁脾虚型模型大鼠组织中SP及肠道敏感性的影响,探讨隔药饼灸治疗FGIDs的可能的作用机制.方法:60只SD大鼠按照随机数字表法随机分为空白组、模型组、隔药饼灸组、逍遥散组和多潘立酮组5组,每组12只,除空白组外其余4组采用复合病因造模法(慢性束缚应激+过度疲劳+饮食失节+夹尾+摇晃)造模,连续21 d;再按被试因素施加方法每天进行隔药饼灸和逍遥散、多潘立酮灌胃14 d.分组治疗2wk后,采用腹壁撤离反射检测肠道敏感性,用Western blot法检测下丘脑、脊髓、胃窦和结肠组织中SP的含量.结果:隔药饼灸组、逍遥散组与多潘立酮组大鼠的肠道注水量明显高于模型组,SP含量明显低于模型组,差异均有高度统计学意义(P<0.01)(肠道注水量:0.8308 mL±0.2364 mL,0.9217 mL±0.1752 mL,0.8000 mL±0.1924mL vs 0.5775 mL±0.1103 mL,下丘脑中的SP含量:0.6780±0.0220,0.6840±0.0288,0.6933±0.0278 vs 0.8998±0.0239;脊髓中的SP含量:0.4430±0.0393,0.4763±0.0308,0.4628±0.0185 vs 0.5563±0.0261;胃窦中的SP含量:0.5690±0.0292,0.5546±0.0261,0.5583±0.0163 vs 0.6821±0.0224;结肠中的SP含量:0.5556±0.0209,0.5705±0.0282,0.5823±0.0361 vs 0.7477±0.0210,均P<0.01),但三组间比较差异无统计学意义(P>0.05).结论:隔药饼灸能和逍遥散、多潘立酮一样通过下调各组织中SP的含量,从而达到改善肠道敏感性的作用,这可能是其治疗FGIDs的另一作用机制.
AIM： To observe the influence of herbal cakeseparated moxibustion on the expression of substance P （SP） and intestinal sensitivity in rats with functional gastrointestinal disorders due to syndrome of liver stagnation and spleen deficiency and to explore the possible mechanism underlying the therapeutic effects of herbal cake-separated-moxibustion against functional gastrointestinal disorders. METHODS： Sixty SD rats were randomly and equally divided into five groups： blank group, model group, herbal cake-separatedmoxibustion group, Xiaoyaosan group and domperidone group. Except for the blank group, the other four groups underwent modeling by applying chronic restraint stress ＋ excessive fatigue ＋ irregular food ＋ tail clipping ＋ shaking for 21 consecutive days. The herbal cake-separatedmoxibustion group underwent herbal cake-separated moxibustion for 14 d, while the Xiaoyaosan group and domperidone group were given Xiaoyaosan and domperidone by gavage for the same duration, respectively. At the end of the experiment, intestinal sensitivity was determined by intestinal water injection rate when the abdominal withdrawal reflex score was 3 points, and the expression of SP in the tissues of the hypothalamus, spinal cord, gastric antrum and colon were measured by Western blot. RESULTS： The intestinal water injection rate was significantly higher in the herbal cake-separated moxibustion group, Xiaoyaosan group and domperidone group than in the model group （0.8308 mL ± 0.2364 mL, 0.9217 mL ±0.1752 mL, 0.8000 mL ±0.1924 mL vs 0.5775 mL ± 0.1103mL, P 〈 0.01 for all）, while the expression of SP （relative grey value ） in the tissues of the hypothalamus, spinal cord, gastric antrum and colon was significantly lower in herbal cake-separated moxibustion group, Xiaoyaosan group and domperidone group than in the model group （hypothalamus： 0.6780 ± 0.0220, 0.6840 ± 0.0288, 0.6933 ± 0.0278 vs 0.8998 ±0.0239; spirtal cord： 0.4430 ±0.0393, 0.4763 ±0.0308, 0.4628 ± 0.0185 vs 0.5563 ±0.0
出处
《世界华人消化杂志》
CAS
北大核心
2014年第12期1682-1687,共6页
World Chinese Journal of Digestology
基金
国家973计划理论专项课题基金资助项目
No.2009CB522904
国家自然科学基金课题基金资助项目
Nos.81173326
81303049
长沙市科技计划重点基金资助项目
No.K1005020-31
湖南省高校创新平台开放基金资助项目
No.12K087
高等学校博士学科点专项科研基金资助项目
No.20124323110001
湖南省科技计划基金资助项目
No.2011SK3095
湖南省中医药管理局基金资助项目
No.2013117~~
关键词
隔药饼灸
功能性胃肠病
肝郁脾虚模型
SP
肠道敏感性
Herbal cake-separatedmoxibustion
Functional gastrointestinal disorders
Liver-qi stag-nation and spleen-qi deficiency
SP
Intestinal sensitivity
