摘要
用 PCR技术 ,从水肿病大肠杆菌临床分离株中扩增出 F1 0 7A亚单位基因 ( fed A)的 51 0 bp的全序列 .将该 PCR扩增产物在 Bam H 和 Eco R 位点克隆进 p UC1 8质粒载体 ,并转化大肠杆菌 TG1,再根据限制性内切酶酶切分析筛选出含有 fed A的重组质粒 pf1 0 7G.将重组质粒进行序列测定 ,结果表明 :此重组质粒中的插入序列与发表的 fed A是一致的 ,证明该重组质粒就是含有 fed
The fedA gene (510 bp) of the fimbriae F107 was amplified from the genomic DNA of E. coli field isolate G by polymerase chain reaction (PCR). The PCR product of fedA was cloned into pUC18 at the EcoR Ⅰ and BamH Ⅰ sites. Restriction endonucleases analysis was used to identify the recombinant plasmid, and the recombinant plasmid pf107G was obtained. The pf107G sequence was analysed, and it was in accord with the published sequence of fedA. This demonstrated that the recombinant plasmid pf107G contained the fedA gene.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
2001年第1期41-43,共3页
Journal of Yangzhou University：Natural Science Edition
基金
江苏省自然科学基金!资助项目 (BK95 0 8730 3)
