摘要
目的探讨芬太尼联合丙泊酚作用于大鼠肺缺血再灌注损伤(I/R)模型后,对核因子-κB(nuclear factor-κB)表达的影响及其机制。方法将40只雄性Wistar大鼠随机分为5组(n=8):假手术组(S组)、缺血再灌注损伤组(I/R组)、芬太尼干预组(F组),丙泊酚干预组(P组),芬太尼联合丙泊酚干预组(F+P组)。对大鼠动脉血进行血气分析;检测大鼠肺组织湿/干(W/D)值;对大鼠肺组织进行大体形态学及HE染色后镜下病理学观察,采用免疫组化法测定大鼠肺组织NF-κB表达水平的变化。结果与I/R组比较,F组、P组以及F+P组大鼠肺组织核因子-κB表达明显降低,差异有统计学意义(P<0.01);大鼠动脉血PaO2值升高,差异有统计学意义(P<0.01);大鼠肺组织W/D值明显降低,差异有统计学意义(P<0.01)。I/R组肺毛细血管扩张充血,肺泡间质水肿并有炎性细胞浸润,肺泡腔内可见炎性细胞渗出。假手术组(S组)、芬太尼干预组(F组),丙泊酚干预组(P组),芬太尼联合丙泊酚干预组(F+P组)在肺泡结构较完整性,减轻肺间质及肺泡腔渗出、水肿肺组织保护方面均较I/R组明显减轻。结论芬太尼联合丙泊酚干预可显著减轻大鼠肺缺血再灌注损伤,可能是芬太尼联合丙泊酚能降低大鼠肺组织NF-κB水平有关。
Objective To study the expression of nuclear factor-KB（NF-KB） in the lung and plasma separately and ex- plore the possible mechanisms of fentanyl combine with propofol on lung ischemia-reperfusion injury of rats. Methods The 40 Wistar male rats weighing 220 - 270 g were divided randomly into five groups which were pretreated differently. The values of NF-KB in rats＇ lung was detected by using immunohistochemical method, the lung tissue observed in vitro and under the microscope after HE staining, the rats＇ arterial oxygen pressure and the lung tissue W / D ratio were measured in each group. Results Compared with the I / R group, the F group, the P group, and the F＋P group all followed the same trend： the NF-KB expression levels were obviously lower （P〈0.01）, the values of Pa02 were obviously higher（P〈0.01）, and the W / D ratios were lower（P〈0.01）. Conclusion Fentanyl combined with p1＂opofol can significant- ly reduce lung ischemia-reperfusion injury, it may be that fentanyl combined with propofol can reduce the level of NF- KB in rats＇ lung.
出处
《中国现代医生》
2014年第15期10-13,共4页
China Modern Doctor
关键词
芬太尼
丙泊酚
缺血再灌注损伤
Fentanyl
Propofol
Nuclear factor-KB
Ischemia reperfusion injury
