摘要
目的探讨青蒿琥酯(Art)对高糖诱导下的大鼠肾小管上皮细胞(NRK-52E)Toll样受体4(TLR4)、核转录因子-κB(NF-κB)表达及合成的影响。方法体外培养NRK-52E细胞,分为6组:正常对照组、高糖组、Art小剂量组、Art中剂量组、Art高剂量组、依那普利组。培养48 h后收集各组细胞,采用逆转录-聚合酶链反应(RT-PCR)法检测细胞TLR4、NF-κB mRNA含量;采用Western blot法检测细胞TLR4、NF-κB蛋白表达水平。结果 1与正常对照组比较,高糖组细胞TLR4、NF-κB mRNA和蛋白表达水平明显升高(P<0.05);2与高糖组比较,Art小、中、高剂量组细胞TLR4、NF-κB mRNA和蛋白表达水平明显降低,且呈剂量依赖性(P<0.05);3与依那普利组比较,Art小、中、高剂量组细胞TLR4水平表达均升高,且Art高剂量组与依那普利组差异无统计学意义。与依那普利组比较,Art小、中剂量组细胞NF-κB水平表达升高,Art高剂量组细胞NF-κB水平表达降低,且Art中、高剂量组与依那普利组差异无统计学意义。结论 Art可呈剂量依赖性地抑制高糖环境下NRK-52E细胞TLR4、NF-κB的高表达及合成。
Objective To explore the effects of artesunate （ Art） on expression and synthesis of Toll-like receptor-4 （TLR4） and nuclear factor-κB （NF-κB） in rat renal tubular epithelial cells （NRK-52E） induced by high glu-cose. Methods NRK-52E cells were cultured and divided into six groups： normal control group, high glucose group,Art in low dose group,Art in medium dose group, Art in high dose group, and Enalapril group. Cells were collected after 48 hours. The expressions of TLR4 and NF-κB at mRNA and protein levels were detected by RT-PCR and Western blot. Results ①Compared with the normal control group, the expressions of TLR4 and NF-κB at mRNA and protein in high glucose group gradually increased （ P〈0. 05 ）;② Compared with the high glucose 〈br〉 group, as increases in Art doses, the expressions of TLR4 and NF-κB at mRNA and protein in the Art in low, me-dium and high dose groups gradually reduced （P〈0. 05）;③Compared with the Enalapril group, the expressions of TLR4 mRNA and protein in the Art in low, medium and high dose groups increased, and the difference between the Art in high dose group and the Enalapril group had no statistical significance （ P〉0. 05 ） . Compared with the Enalapril group, the expressions of NF-κB mRNA and protein in the Art in low and medium dose groups increased, but decreased in the Art in high dose group. Furthermore, there was no statistical difference in the Art in medium and high dose groups when compared with the Enalapril group（P〉0. 05）. Conclusion Art can suppress the high expression and synthesis of TLR4 and NF-κB of NRK-52 E cells induced by high glucose in a dose-dependent man-ner.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第7期904-908,共5页
Acta Universitatis Medicinalis Anhui
基金
广西医疗卫生适宜技术研究与开发项目(编号:S201316-07)
广西自然科学基金项目(编号:2012GXNSFAA053085)
广西高等学校计划科研项目(编号:201204LX245)
