摘要
目的对海洋芽孢杆菌B-9987中macrolactin生物合成基因簇及其关键基因进行分析、鉴定及功能研究。方法通过生物信息学手段对基因簇的基因组成和功能结构域进行了分析;构建了用于酰基转移酶基因高表达的大肠杆菌—芽孢杆菌穿梭载体,采用电击转化方法导入B-9987之中进行高表达。结果 Macrolactins是由位于同1个操纵子的9个基因bmmA-I所编码的反式酰基转移酶聚酮合酶体系组装而成,反式酰基转移酶(trans-acyltransferase,trans-AT)BmmA的高表达使macrolactin A的产量提高了约0.6倍。结论 Macrolactin生物合成基因簇在具有生物防治活性的芽孢杆菌中普遍存在,且高度保守;反式酰基转移酶的高表达能够增加macrolactin A的产量。
Objective To identify and characterize the macrolactin gene cluster from Bacillus rnarinus I3- 9987. Methods By elaborate bioinformatics analysis, the gene organization and functional domain composition of the macrolactin gene cluster were predicted; for overexpression of the trans-acyl transferase （trans-AT） gene, an E. colffBacillus shuttle vector was constructed and introduced into B. marinus 13- 9987 by electroporation. Results Macrolactins were assembled via a trans-AT polyketide synthase sys- tem, which was encoded by 9 genes, brnrnA-I, located in an operon; overexpression of trans-AT BrnrnA led to the improved macrolactin A production by about 0. 6-fold. Conclusion Macrolactin biosyn- thetic gene clusters are highly conserved among biocontrol Bacillus strains; overexpression of trans-acyl transferase BmmA is able to increase macrolactin A production.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2014年第3期69-75,共7页
Chinese Journal of Marine Drugs
基金
国家自然科学基金项目(31070072
31171201)
教育部新世纪优秀人才支持计划项目(NCET-09-0717)资助
