摘要
目的研究树突状细胞及其细胞因子在多囊卵巢综合征患者在卵泡液中的表达及其临床意义。方法收集多囊卵巢综合征患者取卵日卵泡标本30例,以因男方因素行体外受精-胚胎移植30例患者作为对照组;应用流式细胞术检测树突状细胞的分布,酶联免疫吸附试验检测细胞因子TNF-α、IL-6、IL-10和IL-23等表达水平,并分析HLA-DR平均荧光强度、TNF-α浓度与血雌二醇水平的关系。结果与对照组标本(21.27%±5.5%)相比,多囊卵巢综合征患者CD11c+HLA-DR+DC细胞显著减少(16.22%±5.5%);hCG日血雌二醇水平与DC活化标志HLA-DR平均荧光强度呈正相关(r=0.75,P<0.01),与卵泡液中TNF-α浓度水平呈负相关(r=-0.69,P<0.01)。与正常对照组相比,PCOS患者卵泡液TNF-α、IL-6和IL-10的浓度显著增多,而致炎性细胞因子IL-23浓度显著降低。结论 PCOS患者卵泡微环境呈现免疫抑制状态,与卵泡发育障碍可能具有相关性。
Objective To investigate the expression of dendritic cells （DC） in follicular fluid of polycystic ovarian syndrome （PCOS） patients and its clinical significance. Methods The samples of follicular fluid were collected from PCOS patients at the day of transvaginal oocyte retrieval （n=30）. Patients with severe male factor infertility were set as control group （n=30）. Phenotypes of DC were detected by flow cytometer and cytokines （TNF-α, IL-6, IL-10 and IL-23） in follicular fluid were assessed by the enzyme-linked immunosorbent assay （ELISA）. Results A significant decrease of the percentage （16.22%±5.5%） of DC was found in PCOS follicular fluid compared with control （21.27%±5.5%）. The mean fluorescence intensity of HLA-DR, which reflects DC maturation, correlated positively with serum level of E2 on the day of hCG administration （r=0.75, P〈0.01）. Serum E2 has a reverse correlation with the concentration of TNF-α in follicular fluid （r=-0.69, P〈0.01）. The level of cytokines （TNF-α, IL-6 and IL-10） increased significantly but proinflammatory cytokine IL-23 decrease in follicular fluid. The percentage of blood Treg cells increased significantly in carcinoma group compared with normal group but less than that of cancerous tissues in bladder carcinoma. Conclusion The decrease of follicular fluid DC from PCOS patients indicates an immunosuppressive regulatory profile in the microenvironment of ovarian follicle, which is associated with the dysfunction of folliculogenesis.
出处
《中华临床医师杂志（电子版）》
CAS
2014年第8期26-29,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
国家自然科学基金青年项目(81300530)
深圳市科技计划项目(医疗卫生类201203043)
