摘要
目的:研究低氧条件下,Ezrin蛋白对乳腺癌细胞增殖、黏附能力及细胞骨架表达的调节作用。方法:用MTT方法检测常氧或低氧条件下,Ezrin对乳腺癌原代细胞增殖、黏附、侵袭的影响;免疫荧光染色法检测常氧或低氧条件下Ezrin对乳腺癌细胞骨架、细胞形态及细胞表面微绒毛表达的影响。结果:常氧或低氧条件下,Ezrin蛋白对乳腺癌细胞增殖均有抑制能力(P常氧<0.05,P低氧<0.05)。黏附能力检测结果显示,在常氧条件下Ezrin蛋白对乳腺癌细胞黏附能力没有明显影响(P常氧>0.05),但是低氧条件下对乳腺癌细胞黏附能力有增强作用(P低氧<0.05)。在对细胞骨架、细胞形态变化及细胞微绒毛表达实验中,观察到Ezrin在低氧条件下可加强细胞表面微绒毛表达及细胞形态变化。结论:低氧条件下,Ezrin蛋白对乳腺癌细胞的黏附能力有影响,对细胞骨架表达、细胞形态改变和细胞表面微绒毛表达的调节作用较为显著。
Objective： To research the regulatory effect of Ezrin protein on proliferative ability and adhesive ability of breast cancer cells and cytoskeleton remodeling under hypoxia. Methods： MTT assay was used to analyze the effect of Ezrin protein on cell proliferation,adhesion and invasion of breast cancer primary cells under normoxia and hypoxia; immunofluorescent staining was performed to detect the effect of Ezrin protein on breast cancer cytoskeleton,cellular morphology and microvilli expression on the cell surface under normoxia and hypoxia. Results： Ezrin protein had inhibiting effect on proliferation of breast cancer cells under normoxia and hypoxia（ Pnormoxia 0. 05,Phypoxia 0. 05）. The results of adhesive ability test showed that the impact of Ezrin protein on adhesive ability of breast cancer cells under normoxia was not significant（ Pnormoxia 0. 05）,but the impact of Ezrin protein on adhesive ability of breast cancer cells under hypoxia strengthened（ Phypoxia 0. 05）. Ezrin protein could enhance microvilli expression on the cell surface and cellular morphological change under hypoxia. Conclusion： Under hypoxia,Ezrin protein can enhance the adhesive ability of breast cancer cells,and its regulatory effect on cytoskeleton remodeling,morphological change and microvilli expression of breast cancer cells are significant.
出处
《中国妇幼保健》
CAS
北大核心
2014年第16期2602-2605,共4页
Maternal and Child Health Care of China
关键词
EZRIN蛋白
低氧
乳腺癌细胞增殖
细胞黏附侵袭
Ezrin protein Hypoxia Proliferation of breast cancer cell Cell adhesion and invasion
