摘要
目的：探讨白藜芦醇（resveratrol,Res）对肺腺癌A549细胞的作用及机制。方法：用不同浓度Res处理A549细胞24h后,倒置显微镜观察细胞形态学变化,DAPI细胞核染色后荧光显微镜观察细胞凋亡特征,MTT检测Res对于A549细胞生长的抑制作用,蛋白质印迹法检测Res作用A549细胞后p53、Bax、Bcl-2和Cleaved-Caspase3蛋白的表达变化。结果：Res处理A549细胞后,细胞间隙变大,细胞核裂,随着药物浓度的增加上述形态变化明显;同时DAPI染色结果提示,Res诱导A549细胞凋亡。25～200μmol/L浓度的Res可明显抑制A549细胞的增殖,25μmol/L时细胞抑制率为12.4%,50μmol/L时为21.6%,100μmol/L时为51.3%,200μmol/L时为89.7%,具有剂量依赖性。24h的IC50为98μmol/L。而且细胞内的p53、Bax和Cleaved-Caspase3蛋白的表达量升高,Pearson相关系数r值分别为0.930和0.874,P值均〈0.001;Bcl-2蛋白被抑制,Bcl-2/Bax比值下降,Pearson相关系数r=0.753,P=0.01。结论：Res抑制A549细胞增殖并通过p53途径诱导A549细胞凋亡,Bax、Bcl-2和Cleaved-Caspase3参与了凋亡过程。
OBJECTIVE：To investigate the function of resveratrol（Res）and its mechanism in lung adenocarcinoma A549cells.METHODS：A549cells were treated with resveratrol at different concentretions for 24hand the morphological change was observed by phase contrast microscope.Apoptosis features were observed by fluorescence microscope after nuclear DAPI staining.The proliferation of A549cells was analyzed using methyl thiazolyl tetrazolium（MTT）assay.The expression of p53,Bax,Bcl-2and Cleaved-Caspase3after Res treatment in A549cells was measured by Western blot.RESULTS：After Res treatment,we found that the interspace of A549cells was decreased and granular material was found in nucleus.These changes were remarkably associated with the increase of resveratrol concentration.DAPI staining indicated that Res induces apoptosis in A549cells.After treated with 25-200μmol/L Res,the proliferation was markedly inhibited with a does dependent manner（25μmol/L,12.4%;50μmol/L,21.6%;100μmol/L,51.3%;200μmol/L,89.7%）,and IC50 was 98μmol/L.Otherwise,after Res treatment,the expression of p53,Bax and Cleaved-Caspase3protein was upregulated（r=0.930,P0.001;r=0.874,P0.001）,Bcl-2and the ratio of Bcl-2/Bax were downregulated（r=0.753,P= 0.01）.CONCLUSION：Resveratrol could inhibit the proliferation and induce apoptosis through p53method in A549cells, and the expression of Bax,Bcl-2and Cleaved-Caspase3participate in the apoptotic process modulated by resveratrol.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2014年第10期740-743,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
白藜芦醇
肺腺癌
增殖
凋亡
resveratrol
lung adenocarcinoma
proliferation
apoptosis
