摘要
目的建立一种新型兔胫骨在体力学加载模型，为组织工程支架原位成骨的实验研究提供依据。方法6个月龄新西兰兔10只，雌雄不限，体质量2．5～3．0kg。采用新西兰兔制备直径3．2mm的胫骨孔状缺损，植入组织工程支架材料，并于缺损两端约1．4cm处行克氏针穿刺术．用于施加压缩载荷：选择1只兔胫骨标本进行Micro—CT断层扫描，采用有限元Mimics10．01软件建立仿真模型，于上位克氏针两端施加压缩应变（ε1）0．5000με，计算缺损部位整体应变ε2，拟合ε1-ε2关系曲线。术后10d处死3只实验动物，取材手术侧胫骨，进行离体标本加载实验，验证有限元分析的结果；术后2周对其余实验动物术侧胫骨施加正弦压缩载荷（10Hz，1000με，10min／3d），持续2周，检测动物模型的可行性。结果有限元分析结果可以为在体加载提供数据支持．标本实验验证了有限元分析的可靠性；参照有限元结果，该在体加载模型可以准确地控制加载参数，并且动物实验表明．该模型制备稳定，动物损伤较轻，具有大规模开展实验的可行性。结论通过有限元建模与标本实验相结合是建立动物实验模型的一种有效手段．在体加载模型能够为力学因素干预组织工程支架原位成骨的相关实验研究提供支持。
Objective To establish a novel type of in vivo loading model on rabbit tibia and provide the evidence for bone tissue engineering scaffold in situ experimental research. Methods Ten New Zealand rabbits of 6-month-old were enrolled in the present study. Porous tibia defects of 3.2 mm diameter were prepared and then implanted with tissue engineering scaffolds, which were punctured with Kirschner wires about 1.4 cm from both ends of the defects for applying compressive load. One rabbit tibia was scanned by Miero-CT and the image data was processed by software to build finite element model for mechanical analysis, the Kirschner compressive strain（ε1 = 0 - 5 000 με） was applied on the upper ends, the overall defect strain ε2 was calculated, and the ε1 - ε2 curve was fitted. Three animals were sacrificed 10 days after operation, and in vitro loading on the operated side tibia specimens was conducted to verify the results of finite element analysis. Two-week after surgery, the operative tibias of the remaining animals were loaded for 2-week（10 Hz, 1 000 με, 10 min/3 d） to test the feasibility of animal model. Results The results of finite element analysis provided data to support the model of in vivo loading, and the specimen experiment verified the reliability of finite element analysis. For finite element results, the in vivo loading parameters could be accurately controlled. The animal experiments had the feasibility of conducting large-scale experiments because of the stability and less damage of the model. Conclusion It is demonstrated that the finite element analysis combined with the specimen experiments is effective to establish an animal model and the in vivo loading model could provide support to the related research of bone formation inside tissue engineering scaffolds affected by mechanical stimulation.
出处
《生物医学工程与临床》
CAS
2014年第3期205-208,共4页
Biomedical Engineering and Clinical Medicine
基金
国家自然科学基金面上项目(11072266)
国家自然科学基金面上项目(31370942)
关键词
胫骨
骨缺损
在体加载
动物模型
tibia
bone defect
in vivo loading
animal model
