摘要
目的:预测人IL-37b蛋白质的二级结构及B细胞抗原表位。方法:以IL-37b蛋白质的氨基酸序列为基础,应用ProScale、Bcepred服务器,分析人IL-37b亲水性、柔韧性、可及性和抗原性指数,并结合二级结构特征,预测IL-37b的B细胞表位。结果:IL-37b的二级结构主要由β折叠(31.65%)、无规则卷曲(52.75%)、α螺旋(8.26%)和β转角(7.34%)组成。其B细胞表位可能位于第21-27、34-75、175-192和213-215氨基酸区段。结论:此结果将有助于确定IL-37b的B细胞表位,为研制人IL-37单克隆抗体提供了理论依据。
Objective：To predict the secondary structure and B-cell epitope of human IL-37.Methods：Based on IL-37b ami-no acid sequence, the secondary structure was predicted by SOPMA; hydrophilicity, flexibility, accessibility index were predicted by software of ProScale, Bcepred, respectively.Combined the results according to these methods , the B cell epitopes of IL-37b were pre-dicted.Results： The second structure of IL-37b contained extended strand （31.65%）, random coil （52.75%）, alpha helix （8.26%）, beta turn （7.34%） and the most possible epitopes of IL-37b were located in or adjacent to amino acid 21-27, 34-75, 175-192 , 213-215 .Conclusion：These results will be helpful for the estimate of the epitopes and provide a theory basis for developing mon -oclonal antibodies against human IL-37.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第5期648-650,653,共4页
Chinese Journal of Immunology
基金
浙江省自然科学基金(LY13H090014)
浙江省教育厅科研项目(Y201327689)
宁波大学学科项目(XKL11D2112)
宁波市科技计划项目(2013C50054)
宁波大学创新性开放实验项目(cxxkf2013-016)资助
