摘要
为进一步开展人白细胞介素Ⅱ（ＩＬ２）基因修饰的肿瘤疫苗的研究，在已构建的人白细胞介素ⅡｃＤＮＡ表达载体（ｐｃＤＮＡ３／ＩＬ）的基础上，利用脂质体，将其引入人肝癌细胞株７７２１，通过Ｇ４１８ 抗性筛选获得阳性克隆，用活细胞计数法检测阳性克隆的细胞培养上清中的ＬＩ２ 活性，结果表明ｐｃＤＮＡ３／ＩＬ２ 转染的７７２１ 细胞所分泌的ＩＬ２ 水平明显高于阳性对照ｐＢｃ１２／ＨＩＶ／ＩＬ２ 转染的７７２１ 细胞所分泌的ＩＬ２ 水平。说明重组表达载体ｐｃＤＮＡ３／ＩＬ２ 的表达效率高于ｐＢｃ１２／ＨＩＶ／ＩＬ２，这为下一步制备新型瘤苗打下了基础。
To explore the research of genetically modified tumor vaccine (GMTV) if interleukin?2(IL?2) in detail,constrcted IL?2 cDNA expression vectors (pcDNA3/IL?2)were transfected into hepatomatic cell strain 7721 by the use of a simplified gene delivery system based on liposome.After culture with selective G418 media,positive cell clones were subcultured.The suspension collected was then added to media for CTLL?2 cell line,a IL?2 dependent cell line,to determine the activity of IL?2 secreted by positive cell clones through calculating living cell numbers.The result suggest the expression of pcDNA3/IL?2 is more efficient than positive control of pBC12/HIV/IL?2 by ATCC.
出处
《中国优生与遗传杂志》
1999年第5期19-19,21,共2页
Chinese Journal of Birth Health & Heredity
基金
国家自然科学基金项目39400154
