摘要
目的：探讨逍遥散对阿尔茨海默病大鼠海马CA3区PP-2A、GSK-3β表达的影响。方法：将清洁级大鼠随机分为4组，其中模型组、西药组、中药组均采用D-gal 腹腔注射和A-β1～42 peptide 双侧海马注射联合造模，生理组仅采用等体积灭菌生理盐水腹腔及海马注射造模。造模完成后，生理组、模型组均用生理盐水灌胃，西药组和中药组分别用奥拉西坦溶液和逍遥散煎剂灌胃，四组灌胃体积均为5 ml/kg，每日1次，连续28 d。灌胃结束后即分离鼠脑，分组标记放入4℃4％戊二醛固定液玻璃容器密封，4℃保存。修块，切取海马区组织，石蜡包埋，切片。 PP-2A稀释度为1∶200，GSK-3β稀释度为1∶150。图像采用Image-pro Plus 5．0分析系统分析，数据采用SPSS11．5统计软件分析，4组间比较用单因素方差分析，组间两两比较采用LSD-t检验，检验水准α＝0．05。结果：PP-2A阳性细胞数、平均光密度及阳性面积、积分光密度等指标的表达，在逍遥散灌胃干预后与模型组比较明显增加（P＜0．01）；GSK-3β以上各项指标在经逍遥散灌胃后较模型组明显减少（P＜0．01）。结论：逍遥散可以上调PP-2A表达和下调GSK-3β表达，可能对凝聚态A-β1～42 peptide诱导的海马Tau蛋白过度磷酸化有一定的抑制作用。
Objective：To study the effect of Xiaoyao Powder on Alzheimer＆#39;s disease in hippocampal CA 3βregion of rats PP-2A,GSK-3βexpression.Methods：Rats were randomly divided into four groups , including model group, western medicine group,Chi-nese medicine group were treated with intraperitoneal injection of D-gal and A-β1-42 peptide bilateral hippocampal injection mold-ing, physiological group only with equal volume of sterile saline intraperitoneal and hippocampal injection molding .The model was completed, normal group, model group were perfused with saline , western medicine group and Chinese medicine group were treated with oxiracetam solution and Xiaoyao decoction , four groups of intragastric volume was 0.5 ml/100 g, 1 time a day, continuous 28 d. After intragastric administration of isolated rat brain immediately , packet marking in 4℃4%glutaraldehyde solution of glass container , stored at 4℃.Repair piece, cut from the hippocampus, embedded in paraffin, sliced.Dilution of PP-2A for 1∶200, GSK-3βdilution of 1∶150.Images were analyzed by using Image-pro Plus 5 image analysis system, data were conducted by SPSS11.5 statistical analysis software, the comparison between 4 groups by single factor analysis of variance , between the two two groups was compared by LSD-t test, the test level of α=0.05.Results：The expression of PP-2A positive cell number and the positive area , average optical density, integral optical density index , in Xiaoyao Powder orally intervention compared with the model group increased significantly （P〈0.01）;GSK-3βabove indices were significantly decreased than that in model group after Xiaoyao Powder after intragastric ad -ministration （P〈0.01）.Conclusion：Xiaoyao Powder can up regulate the expression of PP-2A and down regulate expression of GSK-3β, may be condensed A-β1-42 peptide induced hyperphosphorylation of Tau has certain inhibition .
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第5期623-626,共4页
Chinese Journal of Immunology
基金
河南省2012年度科技计划项目(122102310410)
河南省教育厅青年骨干教师资助项目(2011GGJS-220)
