摘要
目的观察温和灸对大黄所致脾虚证大鼠血浆β-内啡肽的影响,探讨艾灸治疗脾虚证的作用机制。方法将40只健康SD大鼠随机分为4组,即生理盐水温和灸组、生理盐水不灸组,大黄水温和灸组、大黄水不灸组。运用大黄水煎剂灌胃制作脾虚证模型,观察SD大鼠一般状况,使用放射免疫法测定大鼠模型各组血浆β-内啡肽的水平。结果与生理盐水不灸组比较,生理盐水温和灸组血浆β-EP水平明显提高(P<0.01)。与大黄水不灸组和大黄水组比较,大黄水温和灸组血浆β-EP水平明显提高(P<0.01)。与大黄水组比较,大黄水不灸组血浆β-EP水平提高(P<0.05)。结论艾灸足三里、中脘等穴可明显改善实验性脾虚证大鼠的脾虚状况,温和灸法对正常大鼠和脾虚大鼠的血浆β-EP水平均有调节作用,对血浆β-EP水平的调节可能是艾灸治疗脾虚证的作用机理之一,也可能是增强正常个体体质,防治未病的作用机理之一。
Objective To observe the effect of rhubarb induced mild moxibustion;explore the mechanism of moxibustion treatment of spleen deficiency syndrome. Methods 40 healthy SD rats were randomly divided into 4 groups,namely the normal saline and mild moxibustion group、the normal saline without moxibustion group,the rhubarb and mild moxibustion group、the rhubarb without moxibustion group. Water decoction of rhubarb was made by filling the stomach spleen deficiency rat model,observe the general condition of SD rats,determine the level of plasma β-EP endorphin by radio immunoassay. Results compared with the normal saline without moxibustion group,the normal saline and mild moxibustion group’s plasma β-EP levels significantly increased （P〈0. 01）;compared with the rhubarb without moxibustion group and the rhubarb group,the rhubarb and mild moxibustion group’s plasma β-EP levels were significantly increased （P〈0.01）;compared with the rhubarb group,the rhubarb without moxibustion group’s plasma β-EP level increased （P〈0.05）. Conclusions Moxibustion at zusanli、zhongwan point can obviously improve the rat model of spleen deficiency syndrome of spleen deficiency,mild moxibustion has regulatory effect on normal rats and rats with spleen deficiency plasma β-EP levels,the regulation of plasma β-EP levels may be one of the mechanism of Moxibustion in the treatment of spleen deficiency, may also be one of the mechanism of building up normal individuals’constitution and preventing disease.
出处
《云南中医学院学报》
2014年第2期41-43,50,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
湖南省大学生研究性学习和创新性实验计划
