摘要
目的比较具有吸附抗生素能力的2种血培养瓶,即BACTEC PLUS树脂需氧瓶(简称BD-P瓶)与BacT/Alert FA活性炭需氧瓶(简称Bio-FA瓶)对血流感染的检测能力。方法每位患者从两个不同部位采集血标本分别注入BD-P瓶和Bio-FA瓶,放入相应培养仪进行培养,记录报阳时间。结合临床用药资料对这2种具有不同吸附剂的血培养瓶的阳性检出率及报阳时间进行比较。结果在6670瓶血培养中阳性报警共1017瓶,确认污染196瓶(2.94%),有临床意义的阳性821瓶(12.31%)。共检出病原菌493株,其中双瓶阳性334例(67.75%);BD-P瓶单瓶阳性102例(20.69%),Bio-FA瓶单瓶阳性57例(11.56%)。BD-P瓶对病原菌的检出率优于Bio-FA瓶(P<0.01),对于肠杆菌科和葡萄球菌的检出率BD-P瓶明显高于Bio-FA瓶(P<0.01)。BD-P瓶细菌报阳时间早于Bio-FA瓶(P<0.01),特别是对肠杆菌科和非发酵菌的阳性报警时间明显早于Bio-FA瓶。由正在使用药物治疗的患者中检出344例阳性,其BD-P瓶的阳性检出率和报阳时间均高于和快于Bio-FA瓶(P<0.05);按不同的治疗药物进行分组,对头孢哌酮/舒巴坦BD-P瓶的吸附能力更强(P<0.05)。结论应用BD-P瓶检测血流感染较Bio-FA瓶检出率更高,检测速度更快。而联合使用Bio-FA瓶和BD-P瓶,比单用一种培养瓶更具优势。
Objective To compare the detectability to the bloodstream infection by 2 kinds bottles of BACTEC PLUS resin oxygen bottle （BD-P bottles） and BacT/Alert FA active carbon oxygen bottle （Bio-FA bottles）.Methods All blood samples of pa-tients from two different parts were injected into BD-P bottles and Bio-FA bottles and cultured in the blood culture system ,record posi-tive time.The positive rate and positive time of 2 different adsorbent blood culture bottles were compared combining with clinical data . Results There are 1017 positive bottles between the 6670 bottles,196 bottles were confirmed pollution ,accounted for 2.94%;the clin-ical significance of the positive 821 bottles,accounted for 12.31%.493 pathogenic strains were detected ,of which two bottles was posi-tive in 334 patients （67.75%）,single BD-P bottle was positive in 102 cases （20.69%）;single Bio-FA bottle was positive in 57 cases （11.56%）.The detection rate of Bio-FA bottle was higher than that of BD-bottle P （P＆lt;0.01）,especially for the detection of Enter-obacteriaceae and Staphylococcus .BD-P bottle bacteria Bao Yang predates the Bio-FA bottle （P＆lt;0.01）,especially the positive alarm time for Enterobacteriaceae and non fermentive bacteria significantly earlier in Bio-FA bottles.In 344 positive cases using drug treat-ment,the positive rate and time of BD-P were higher and faster than that of the Bio-FA bottle （ P＆lt;0.05）;and the adsorption ability of BD-P bottles to cefoperazone/sulbactam was stronger than that of the Bio-FA bottle （ P ＆lt;0.05 ） according to different therapeutic drugs.Conclusion The detectability of BD-P bottles to the bloodstream infection was higher and faster than that of Bio-FA bottles. Combination of Bio-FA bottle and BD-P bottle was more advantage than using a single bottle .
出处
《东南国防医药》
2014年第2期117-120,共4页
Military Medical Journal of Southeast China
基金
国家青年自然科学基金项目(81302536)
