摘要
目的探讨膜联蛋白Ⅱ(AnnexinⅡ)在支气管肺癌组织、肺泡灌洗液(BALF)和胸腔积液中的表达水平及在肺癌诊断中的价值。方法收集2010年10月—2011年10月兰州大学第一医院呼吸内科收治的肺癌伴胸腔积液患者60例(试验组)以及结核性渗出性胸腔积液患者60例(对照组)。对肺癌组织蜡块标本保留完整的48例患者的肺癌组织进行免疫组化染色,并收集该院病理科提供的18例正常肺组织蜡块标本,检测AnnexinⅡ在肺癌组织及正常肺组织中的阳性表达情况;应用酶联免疫吸附试验(ELISA)法检测试验组及对照组患者的BALF、胸腔积液中AnnexinⅡ的表达水平。结果肺癌组织中AnnexinⅡ的阳性表达率(62.5%,30/48)高于正常肺组织(16.6%,3/18),差异有统计学意义(P<0.05)。AnnexinⅡ在肺癌组织中的表达与患者的性别、吸烟与否、癌细胞分化程度及病理分型无关(P>0.05);与病理分期以及淋巴结转移有关(P<0.05)。试验组患者BALF、胸腔积液中AnnexinⅡ的表达水平高于对照组(P<0.05)。两组BALF中AnnexinⅡ的表达水平分别高于各自组内胸腔积液中AnnexinⅡ的表达水平(P<0.05)。结论 AnnexinⅡ在肺癌组织中的阳性表达率及在肺癌患者BALF、胸腔积液中的表达水平均较高,故AnnexinⅡ可作为肺癌诊断的良好标记物。
Objective To assess the diagnostic value of annexin n in the diagnosis of lung cancer （LC） by detennining Annexin n expression in LC tissue, bronchoalveolar lavage fluid （BALF） , pleural effusion （PE）. Methods From October 2010 to October 2011, 60 LC patients with PE （study group） and 60 patients with tuberculous exudative PE （control group） admitted to Department of Respiratory Medicine, the First Hospital of Lanzhou University were enrolled in this study. Immunohisto chemical method was performed on the samples of 48 patients with fully retained paraffin block LC tissue, and 18 cases of normal lung tissue paraffin block samples were collected, to determine the positive expression of Annexin Ⅱ in LC or normal tissues. BALF, PE, Annexin Ⅱ expressions were detected by enzyme limed immunosorbent assay （EUSA）. Results Annexin n positive expression rate was higher in LC tissues （62.5%, 30/48） than in normal lung tissues （16.6%, 3/18）, the difference was significant （P 〈 0. 05 ）. Expression of Annexin n in LC tissues was not associated with gender, smoking, differentiated degree of cancer cells or pathological typing （P 〉 0. 05）, but associated with pathological staging, lymph node metastasis （P 〈 0. 05）. The expressions of Annexin Ⅱ in BALF, PE were higher in study group than in control group （P 〈 0. 05）. The expression of Annexin n was higher in BALF than in PE in 2 groups （P 〈 0. 05）. Conclusion The positive expression rate of Annexin Ⅱ in LC tissue and in patients with LC BALF, PE expression levels were higher, so Annexin Ⅱ can be used as a good marker for diagnosis of LC.
出处
《中国全科医学》
CAS
CSCD
北大核心
2014年第11期1307-1310,共4页
Chinese General Practice
基金
甘肃省科技厅技术研究与开发专项计划项目(1105TCYA012)
关键词
肿瘤标记物
膜联蛋白Ⅱ
支气管肺泡灌洗液
胸腔积液
癌
非小细胞肺
Tumor marker
Annexin Ⅱ
Bronchoalveolar lavage fluid
Pleural effusion
Carcinoma, non - small - cell lung
