摘要
目的研究灵芝多糖对糖尿病(DM)模型大鼠肾脏保护作用及分子机制。方法建立DM大鼠模型,分为对照组、模型组和药物治疗组,药物治疗组用灵芝多糖治疗8周。观察3组大鼠肾功能指标、氧化-抗氧化指标、原位末端标记(TUNEL)法检测肾脏细胞凋亡,Western-blot检测Bcl-2、转化生长因子-β1(TGF-β1)和Bax在肾脏的蛋白表达水平。结果药物治疗组的肾功能指标好于模型组,低于对照组,差异有统计学意义(P<0.05);药物治疗组肾皮质的丙二醛(MDA)低于模型组,高于对照组;超氧化物歧化酶(SOD)高于模型组,低于对照组,差异有统计学意义(P<0.05);药物治疗组肾脏细胞凋亡率低于模型组,高于对照组,差异有统计学意义(P<0.05);药物治疗组肾皮质的Bcl-2蛋白表达水平高于模型组,低于对照组,BAX和TGF-β1蛋白表达水平低于模型组,高于对照组,差异有统计学意义(P<0.05)。结论灵芝多糖可能通过改善DM大鼠氧化应激水平和抑制肾脏细胞凋亡保护DM大鼠的肾功能。
Objective To study the effects of the polysaccharides of Ganoderma lucidum on the kidneys of diabetesmellitus （DM） rats and the related molecular mechanism. Methods The DM rat model was established. The DM rats weredivided into control group, model group and treatment group. The treatment group was treated by Ganoderma lucidumpolysaccharides for 8 weeks. The rat renal function profile, oxidation and anti-oxidation index were determined. TUNEL assaymethod was used for the detection of renal cell apoptosis. The expression levels of Bcl-2, TGF-β1 and Bax in the kidney weredetermined by Western blotting. Results After treatment with Ganoderma lucidum polysaccharide, renal function profile in thetreatment group was significantly better than that in the model group, and worse than that in the control group （both P〈0. 05）.Renal cortical MDA of treatment group was significantly lower than that of model group, and higher than that of the control group （both P〈0. 05）. SOD of the treatment group was significantly higher than that of the model group, and lower than that of thecontrol group （both P〈0. 05）. Renal cell apoptosis rate of the treatment group was significantly lower than that of the modelgroup, and higher than that of the control group （both P〈0. 05）. Renal cortical Bcl-2 expression level of the treatment group wassignificantly higher than that of model group, and lower than that of the control group （both P〈0. 05）. The expression levels ofBAX and TGF-β1 protein of the treatment group were significantly lower than those in the model group, and higher than those ofthe control group （both P〈0. 05）. Conclusion Ganoderma lucidum polysaccharides may improve oxidative stress and inhibitrenal apoptosis of renal cells to protect the renal function of DM rats.
出处
《医药导报》
CAS
北大核心
2014年第2期144-148,共5页
Herald of Medicine
基金
盐城市医学科技发展计划项目(YK2010112)
关键词
灵芝多糖
糖尿病
氧化应激
凋亡
Ganoderma lucidum polysaccharide
Diabetes mellitus
Oxidative stress
Apoptosis
